Characterization and evaluation of rice straw lignin phenolics on pollen germination in pumpkin
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Date
2021
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Punjab Agricultural University, Ludhiana
Abstract
Rice straw is one of the most abundant, renewable, cost-effective and non-edible agricultural
feedstocks. Its burning has led to air pollution, loss of soil fertility and nutrient depletion,
therefore valorization of rice straw is important. Straw was found to be chemically composed
of 24% hemicellulose, 41% cellulose, 9% lignin, 20% extractives and 6% ash (silica). Lignin
was extracted using dilute acid pretreatment (1% H2SO4) followed by alkaline hydrolysis
using NaOH. FT-IR, 1H and 13C NMR were used for structural characterization of the lignin
obtained from rice straw. The ethyl acetate extract of acid solubilized lignin was found to
contain p-coumaric acid, ferulic acid and caffeic acid as the major phenolic acids which were
isolated using preparative thin layer chromatography. The phenolic acids were characterized
using elemental analyses, FT-IR, 1H and 13C NMR spectroscopy. Amides of p-coumaric acid,
ferulic acid and caffeic acid were synthesized by their reaction with propyl and butyl amines
using microwave irradiation and analysed using spectral studies. Phenolic acids and their
amides were evaluated for their effect on pollen germination and tube growth in pumpkin. It
was concluded that at 5 ppm concentration, germination percentage and pollen tube growth of
p-coumaric acid, caffeic acid and their amides was enhanced than the control. These results
could be utilised in increasing pollen tube length of Cucurbita moschata while making
interspecific cross between C. moschata and C. pepo in order to transfer the hull-less and
disease and virus resistant character of C. moschata to C. pepo.
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Jasleen Kaur (2021) Characterization and evaluation of rice straw lignin phenolics on pollen germination in pumpkin (Unpublished M.Sc. thesis). Punjab Agricultural University, Ludhiana, Punjab, India.