IDENTIFICATION OF BOVINE TUBERCULOSIS ASSOCIATED PUTATIVE SNP IN ONGOLE CATTLE AND MURRAH BUFFALO
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Date
2018-01
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SRI VENKATESWARA VETERINARY UNIVERSITY TIRUPATI - 517 502. (A.P.) INDIA
Abstract
ABSTRACT:
Bovine Tuberculosis is a chronic infectious respiratory disease caused by
Mycobacterium bovis. The conventional preventive and control measures are not so
effective in combating the disease, hence alternate strategies like selective breeding of
animals resistant to the disease by exploiting the natural variation of the genes
underlying the disease resistance trait to the disease could be considered. The present
study is aimed at identifying those genes and SNPs to be associated with bTB
susceptibility / resistance and exploiting the genetic variations in the gene regions by
using PCR-RFLP method. Two genes (CCL4 and RNF185) were identified to be
promising candidate genes in association with bTB and their SNPs that create
restriction enzyme sites were considered for the present study. DNA was isolated from
blood samples of 128 Ongole cattle and 155 Murrah buffaloes by modified high salt
method. Two sets of primers were designed so as to capture the SNP regions of the
genes. The PCR products were subjected to RFLP with BtsαI and TseI enzymes for
CCL4 and RNF185 genes respectively. In the population under study, both the genes
were found to be monomorphic. The fixation of allele ‘A’ is revealed in CCL4 gene
with the absence of ‘T’ allele while in RNF185 gene, ‘A’ allele is fixed with absence of
‘G’ allele. Both the loci were found to be monomorphic in both cattle and buffalo.
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