Prevalence and characterization of the Shiga toxin producing Escherichia coli isolated from bovine mastitis

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dc.contributor.advisorMishra, Rajashree
dc.contributor.authorBisht, Kailash Singh
dc.contributor.otherPati, Prasad Kumar
dc.contributor.otherPatra, Ritun
dc.contributor.otherRath, Prasana Kumar
dc.date.accessioned2022-01-31T07:18:50Z
dc.date.available2022-01-31T07:18:50Z
dc.date.issued2020
dc.descriptionTH - 6097en_US
dc.description.abstractThe Shiga toxin producing E. coli is a main food-borne human pathogen threatening public health worldwide. The significance of the STEC associated diseases outbreak continuously increasing over the year and the STEC O157 serogroup is considered as an important animal origin pathogen. It is also recognized as an important etiology of the bovine mastitis which negatively impacts the animal health and dairy industry. As the “one health” approach became more important in recent years. It’s mainly focuses on the zoonotic diseases, environmental contamination and spread of antimicrobial resistance due to the interaction between human, animal and their environment. The current research work was done to determine prevalence and to characterize E. coli and Shiga toxin producing E. coli isolated from bovine mastitis milk. A total of 250 bovine mastitis milk samples i.e., 172 cattle and 78 buffalo samples were tested. On routine microbial examination and biochemical characterization of the bacteria isolated from bovine mastitis milk, 27.20% (n= 68) were positive for E. coli, out of which 10.27% (n= 7) are positive for the STEC O157 using sorbitol MacConkey agar. On virulence factors such as serum resistance, Congo red dye uptake and haemolysin activity showed 60.29%, 30.88% and 10.29% for total E. coli isolates and 85.71%, 71.43% and 42.86% for STEC O157 isolates respectively. Serum resistance was the most common virulence activity shown by the bovine mastitis associated E. coli and STEC O157 isolates. The biofilm production was determined by Congo red agar method, 75 % and 71.43% isolates of total E. coli and STEC O157 showed positive results respectively. By microtiter plate assay E. coli showed 77.94% and 52.94% results, whereas STEC O157 showed 71.43 % and 57.16% results at 20 ˚C and 37 ˚C respectively. Microtiter plate assay method showed more sensitivity for screening of biofilm production compared to Congo red agar method. The antibiotic susceptibility test showed Gentamicin as most effective and Ampicillin as most resistant by planktonic and sessile E. coli. Whereas Gentamicin, Enrofloxacin, Ceftriaxone and Doxycycline were the most effective and Ofloxacin was the least effective antibiotic by planktonic and sessile STEC O157. The multidrug resistance was also evident in the E. coli and STEC O157 isolated from bovine mastitis milk samples. The multidrug resistance is much higher in sessile form of E. coli compared to their planktonic forms.en_US
dc.identifier.urihttps://krishikosh.egranth.ac.in/handle/1/5810181380
dc.keywordsEscherichia coli, Gentamicin, Enrofloxacin, Ceftriaxone and Doxycycline, E.Coli and Shiga toxin producing, Staphylococci spp., coliforms, Enterococci spp.en_US
dc.language.isoEnglishen_US
dc.pages117pen_US
dc.publisherDepartment of Veterinary Microbiology, OUAT, Bhubaneswaren_US
dc.subVeterinary Microbiologyen_US
dc.themeEscherichia coli, Gentamicin, Enrofloxacin, Ceftriaxone and Doxycycline, E.Coli and Shiga toxin producing, Staphylococci spp., coliforms, Enterococci spp.en_US
dc.these.typeM.V.Sc.en_US
dc.titlePrevalence and characterization of the Shiga toxin producing Escherichia coli isolated from bovine mastitisen_US
dc.typeThesisen_US
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