Morphological and molecular characterization of tuberose (Polianthes tuberosa L.) germplasm

Simple item page
dc.contributor.advisorSrivastava, Ranjan K.
dc.contributor.authorBharti, Swapnil
dc.date.accessioned2023-02-14T04:57:05Z
dc.date.available2023-02-14T04:57:05Z
dc.date.issued2022-07
dc.description.abstractThe present investigation was carried out for two years at Model Floriculture Centre, G.B. Pant University of Agriculture and Technology, Pantnagar and Uttarakhand Council of Biotechnology, Haldi with twenty-eight tuberose varieties including sixteen single and twelve double types. The experiment was laid out in randomized block design with three replications with the objective to evaluate the varieties for yield and quality attributes, to assess the amount of genetic variability and to study the diversity analysis using RAPD markers. The morphological studies revealed that among the single types, Arka Nirantara performed better in terms of spikes, bulbs and bulblet yield followed by Kalyani Single and Mexican Single whereas among the double types, Arka Suvasini followed by Swarna Rekha and Pearl Double had maximum spikes, bulbs and bulblet yield. The variability studies revealed that the Phenotypic coefficient of variation was higher than Genotypic coefficient of variation for all the forty characters studied. Higher Phenotypic coefficient of variation and Genotypic coefficient of variation were observed for weight of individual floret, diameter of individual bulb, weight of individual bulb, days to 50 per cent flowering and yield of bulblets. Higher heritability along with high estimates of genetic advance as per cent of mean were registered for weight of individual floret, diameter and weight of individual bulb and days to 50 per cent flowering in single types whereas in double types only for weight of individual floret. Correlation studies indicated that for all the characters studied, the genotypic correlation coefficients were found to be greater than phenotypic correlation coefficients indicating the role of environmental expression. Based on Mahalanobis D2 analysis, all the single varieties were grouped into four clusters with maximum cluster distance between cluster I and III (73.69) and all the double varieties were grouped into V clusters with maximum cluster distance between cluster IV and V (220.60). Molecular analysis using fifteen RAPD markers confirmed morphological studies and grouped twenty-eight tuberose accessions into seven clusters and many sub-clusters based on Jaccard’s similarity coefficients which ranged between 0.499 to 0.875. Therefore, var. Arka Nirantara and Kalyani Single among the single types whereas Arka Suvasini and Swarna Rekha among the double types can be recommended for commercial cultivation.en_US
dc.identifier.urihttps://krishikosh.egranth.ac.in/handle/1/5810193768
dc.keywordstuberose, morphologyen_US
dc.language.isoEnglishen_US
dc.pages308 p.en_US
dc.publisherG. B. Pant University of Agriculture and Technology, Pantnagaren_US
dc.research.problemMorphologyen_US
dc.subFloriculture and Landscapingen_US
dc.themeTuberoseen_US
dc.these.typePh.Den_US
dc.titleMorphological and molecular characterization of tuberose (Polianthes tuberosa L.) germplasmen_US
dc.typeThesisen_US
Files
Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
Swapnil Bharti 52598.pdf
Size:
10.53 MB
Format:
Adobe Portable Document Format
Description:
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Theses