GENETIC ASSOCIATION MAPPING TO IDENTIFY QTLs FOR DROUGHT TOLERANCE AND EXPRESSION ANALYSIS OF SELECTED GENES UNDER DROUGHT ENVIRONMENT IN FINGER MILLET [Eleusine coracana (L.) Gaertn]
| dc.contributor.advisor | Ravikumar, R. L. | |
| dc.contributor.advisor | Ravikumar, R. L. | |
| dc.contributor.advisor | Ravikumar, R. L. | |
| dc.contributor.author | ANTRE SURESH, HARIBHAU | |
| dc.contributor.author | ANTRE SURESH, HARIBHAU | |
| dc.contributor.author | ANTRE SURESH, HARIBHAU | |
| dc.date.accessioned | 2023-01-04T03:28:15Z | |
| dc.date.available | 2023-01-04T03:28:15Z | |
| dc.date.issued | 2022-09-22 | |
| dc.date.issued | 2022-09-22 | |
| dc.date.issued | 2022-09-22 | |
| dc.description.abstract | Although the finger millet is renowned for its drought tolerance, drought stress at reproductive stage dramatically reduces the seed yield. Three hundred and fifty diverse genotypes of finger millet were evaluated for sixteen agro-morphological traits under drought stress (DS) and well watered (WW) conditions over the two years viz. summer 2019 and summer 2020 at GKVK, Bangalore. The DS at initial reproductive stage significantly affected the performance of the genotypes for seed yield and component traits. The genotype and genotype × environment interaction biplot method was used to identify high yielding and stable genotypes under WW (GE-5078, GPU-66), DS (GE-2644, GE-3605) and across WWDS (GE-2644) conditions. Stepwise multiple linear regression and principal component analysis under WW and DS indicated that, total earhead weight per plant and main earhead weight are the important traits for both the environments. Genome-wide SNPs were identified by re-sequencing of 350 genotypes and the marker trait association analysis revealed 49 SNPs under WW and 34 SNPs under DS conditions were associated with different agro morphological and drought tolerance related traits. Six each drought tolerant and drought susceptible genotypes were evaluated in replicated field trial for reproductive stage drought stress tolerance during summer 2021. The DS at reproductive stage showed significant effect on number of pollen grains per anther, pollen sterility, relative water content and chlorophyll content. Drought tolerant genotypes recorded significantly less pollen sterility and higher relative water content compared to susceptible genotypes. Two contrasting genotypes, GE-1234 and GE-4719 were used to study the expression of Sucrose synthase-1 (SUS1), Cell wall invertase (INCW), Invertase-1 (IVR1) and two uncharacterized genes (GRMZM2G162968 and GRMZM5G844309) in the immature anthers under DS. The expression level of SUS1, INCW, IVR1 and GRMZM2G162968 were reduced under DS conditions and the extent of reduction was influenced by the drought tolerance of the genotypes. | en_US |
| dc.description.abstract | Although the finger millet is renowned for its drought tolerance, drought stress at reproductive stage dramatically reduces the seed yield. Three hundred and fifty diverse genotypes of finger millet were evaluated for sixteen agro-morphological traits under drought stress (DS) and well watered (WW) conditions over the two years viz. summer 2019 and summer 2020 at GKVK, Bangalore. The DS at initial reproductive stage significantly affected the performance of the genotypes for seed yield and component traits. The genotype and genotype × environment interaction biplot method was used to identify high yielding and stable genotypes under WW (GE-5078, GPU-66), DS (GE-2644, GE-3605) and across WWDS (GE-2644) conditions. Stepwise multiple linear regression and principal component analysis under WW and DS indicated that, total earhead weight per plant and main earhead weight are the important traits for both the environments. Genome-wide SNPs were identified by re-sequencing of 350 genotypes and the marker trait association analysis revealed 49 SNPs under WW and 34 SNPs under DS conditions were associated with different agro morphological and drought tolerance related traits. Six each drought tolerant and drought susceptible genotypes were evaluated in replicated field trial for reproductive stage drought stress tolerance during summer 2021. The DS at reproductive stage showed significant effect on number of pollen grains per anther, pollen sterility, relative water content and chlorophyll content. Drought tolerant genotypes recorded significantly less pollen sterility and higher relative water content compared to susceptible genotypes. Two contrasting genotypes, GE-1234 and GE-4719 were used to study the expression of Sucrose synthase-1 (SUS1), Cell wall invertase (INCW), Invertase-1 (IVR1) and two uncharacterized genes (GRMZM2G162968 and GRMZM5G844309) in the immature anthers under DS. The expression level of SUS1, INCW, IVR1 and GRMZM2G162968 were reduced under DS conditions and the extent of reduction was influenced by the drought tolerance of the genotypes. | en_US |
| dc.description.abstract | Although the finger millet is renowned for its drought tolerance, drought stress at reproductive stage dramatically reduces the seed yield. Three hundred and fifty diverse genotypes of finger millet were evaluated for sixteen agro-morphological traits under drought stress (DS) and well watered (WW) conditions over the two years viz. summer 2019 and summer 2020 at GKVK, Bangalore. The DS at initial reproductive stage significantly affected the performance of the genotypes for seed yield and component traits. The genotype and genotype × environment interaction biplot method was used to identify high yielding and stable genotypes under WW (GE-5078, GPU-66), DS (GE-2644, GE-3605) and across WWDS (GE-2644) conditions. Stepwise multiple linear regression and principal component analysis under WW and DS indicated that, total earhead weight per plant and main earhead weight are the important traits for both the environments. Genome-wide SNPs were identified by re-sequencing of 350 genotypes and the marker trait association analysis revealed 49 SNPs under WW and 34 SNPs under DS conditions were associated with different agro morphological and drought tolerance related traits. Six each drought tolerant and drought susceptible genotypes were evaluated in replicated field trial for reproductive stage drought stress tolerance during summer 2021. The DS at reproductive stage showed significant effect on number of pollen grains per anther, pollen sterility, relative water content and chlorophyll content. Drought tolerant genotypes recorded significantly less pollen sterility and higher relative water content compared to susceptible genotypes. Two contrasting genotypes, GE-1234 and GE-4719 were used to study the expression of Sucrose synthase-1 (SUS1), Cell wall invertase (INCW), Invertase-1 (IVR1) and two uncharacterized genes (GRMZM2G162968 and GRMZM5G844309) in the immature anthers under DS. The expression level of SUS1, INCW, IVR1 and GRMZM2G162968 were reduced under DS conditions and the extent of reduction was influenced by the drought tolerance of the genotypes. | en_US |
| dc.identifier.other | Th-13294 | |
| dc.identifier.other | Th-13294 | |
| dc.identifier.other | Th-13294 | |
| dc.identifier.uri | https://krishikosh.egranth.ac.in/handle/1/5810191109 | |
| dc.keywords | Finger Millet, Eleusine coracana (L.) Gaertn | en_US |
| dc.keywords | Finger Millet, Eleusine coracana (L.) Gaertn | en_US |
| dc.keywords | Finger Millet, Eleusine coracana (L.) Gaertn | en_US |
| dc.language.iso | English | en_US |
| dc.language.iso | English | en_US |
| dc.language.iso | English | en_US |
| dc.pages | 309 | en_US |
| dc.pages | 309 | en_US |
| dc.pages | 309 | en_US |
| dc.publisher | University of Agricultural Sciences, Bangalore | en_US |
| dc.publisher | University of Agricultural Sciences, Bangalore | en_US |
| dc.publisher | University of Agricultural Sciences, Bangalore | en_US |
| dc.sub | Plant Biotechnology | en_US |
| dc.sub | Plant Biotechnology | en_US |
| dc.sub | Plant Biotechnology | en_US |
| dc.theme | QTLs for drought tolerance and expression analysis of selected genes under drought environment in finger millet | en_US |
| dc.theme | QTLs for drought tolerance and expression analysis of selected genes under drought environment in finger millet | en_US |
| dc.theme | QTLs for drought tolerance and expression analysis of selected genes under drought environment in finger millet | en_US |
| dc.these.type | Ph.D | en_US |
| dc.these.type | M.V.Sc. | en_US |
| dc.these.type | Ph.D | en_US |
| dc.title | GENETIC ASSOCIATION MAPPING TO IDENTIFY QTLs FOR DROUGHT TOLERANCE AND EXPRESSION ANALYSIS OF SELECTED GENES UNDER DROUGHT ENVIRONMENT IN FINGER MILLET [Eleusine coracana (L.) Gaertn] | en_US |
| dc.title | GENETIC ASSOCIATION MAPPING TO IDENTIFY QTLs FOR DROUGHT TOLERANCE AND EXPRESSION ANALYSIS OF SELECTED GENES UNDER DROUGHT ENVIRONMENT IN FINGER MILLET [Eleusine coracana (L.) Gaertn] | en_US |
| dc.title | GENETIC ASSOCIATION MAPPING TO IDENTIFY QTLs FOR DROUGHT TOLERANCE AND EXPRESSION ANALYSIS OF SELECTED GENES UNDER DROUGHT ENVIRONMENT IN FINGER MILLET [Eleusine coracana (L.) Gaertn] | en_US |
| dc.type | Thesis | en_US |