MOLECULAR CHARACTERIZATION OF INDIGENOUS SPECIES OF APPLE
| dc.contributor.advisor | MODGIL, MANJU | |
| dc.contributor.author | VIKRANT | |
| dc.date.accessioned | 2017-05-15T07:28:49Z | |
| dc.date.available | 2017-05-15T07:28:49Z | |
| dc.date.issued | 2014 | |
| dc.description.abstract | ABSTRACT Morphological and SSR analysis among the different biotypes of the wild indigenous species of apple M. baccataand M. sikkimensiswithin the site and between the sites were carried out. Morphological data shows some variation in characters. 17 SSR primers were used to amplify the DNAs of all biotypes maintained at research stations of IARI, Shimla; NBPGR, Shimla and UHF Solan. A total of 39, 37 and 36 alleles were found to be polymorphic with an average of 64.10%, 67.56% and 47.22% polymorphism in above mentioned three sites. Average PIC values were 0.495, 0.466 and 0.391 in the samples of IARI,Shimla; NBPGR, Shimla and UHF Solan respectively. Results showed the sufficient genetic variation among the biotypes. The similarity in the dendrogram divided the biotypes of IARI in two clusters, and one cluster with some subclusters in other two sites. Grouping of biotypes in clusters showed that they are genetically diverse. Same biotype collected from three stations was run on the gels together and analyzed to check if these are clones. It has been found that their SSR profiles were not identical. The dendrogram generated has divided all the biotypes in two clusters with some subclusters. Biotype with same name did not cluster into same group due to low similarity values between them. There is a doubt that some biotypes may be mislabelled or differences may arise due to different year of collections from their respectivecentre of origin and natural mutations. | en_US |
| dc.identifier.uri | http://krishikosh.egranth.ac.in/handle/1/5810011154 | |
| dc.keywords | wild indigenous species of apple M. baccataand M. sikkimensiswithin,Morphological and SSR analysis, biotypes | en_US |
| dc.language.iso | en | en_US |
| dc.publisher | UHF,NAUNI | en_US |
| dc.sub | Biotechnology | en_US |
| dc.subject | null | en_US |
| dc.theme | wild indigenous species of apple M. baccataand M. sikkimensiswithin,Morphological and SSR analysis, biotypes | en_US |
| dc.these.type | M.Sc | en_US |
| dc.title | MOLECULAR CHARACTERIZATION OF INDIGENOUS SPECIES OF APPLE | en_US |
| dc.type | Thesis | en_US |