STUDIES ON CALLUS INDUCTION POTENTIAL IN LOCAL PADDY VARIETIES Oryza sativa L. indica
| dc.contributor.advisor | Bhavani, P. | |
| dc.contributor.author | TATHAGATA CHANDA | |
| dc.date.accessioned | 2023-05-31T16:27:45Z | |
| dc.date.available | 2023-05-31T16:27:45Z | |
| dc.date.issued | 2023-05-31 | |
| dc.description.abstract | Rice (Oryza sativa L.) is an important crop globally and is studied extensively. Indica rice is reported to be recalcitrant for in vitro culture techniques which make it difficult for genetic transformations and molecular studies. The present study is to establish in vitro protocol for indica rice. Surface sterilisation of seeds was done with different sterilizing agents, 0.05% HgCl2 for 1 min was best. MS salts with different combinations of growth hormones, carbon sources were used. Azucena (Japonica) performed best in media containing sucrose and 2,4- D whereas IR64 (Indica) in media containing maltose and a combination of 2,4-D and kinetin. Varietal variations were observed in callus induction and callus growth. IR-30864 showed initiation in 7 days whereas CTH-1 took 15 days. Among 20 varieties, four varieties that performed better are KMP-220, KCP-1, CTH- 3, Paustic-1. The incubation conditions had an impact on callus induction percentage and days to callus induction. Paustic-1 showed the most change in callus induction percentage and days to callus induction doubled in IR- 30864. Bacterial contamination showed no sign of reduction even when seeds were treated for 1 min with streptomycin sulphate before inoculation. CTH-3 showed the highest contamination. Morphogenetic media containing different concentrations of BAP/Kinetin and NAA was used for evaluation. In media containing NAA (0.5mg/l) and BAP (2.5mg/l) KMP- 220, IR-30864, KCP-1, CTH-3, Rathna chudi showed root growth. In all other combinations callus browning and ultimately blackening were observed. A highly efficient and widely used tissue culture system for indica rice will accelerate the application of gene editing and transformation technology in breeding programs. | en_US |
| dc.identifier.other | Th-13665 | |
| dc.identifier.uri | https://krishikosh.egranth.ac.in/handle/1/5810197490 | |
| dc.keywords | CALLUS, PADDY VARIETIES, | en_US |
| dc.language.iso | English | en_US |
| dc.pages | 113 | en_US |
| dc.publisher | University of Agricultural Sciences, Bangalore | en_US |
| dc.sub | Plant Biotechnology | en_US |
| dc.theme | STUDIES ON CALLUS INDUCTION POTENTIAL IN LOCAL | en_US |
| dc.these.type | M.Sc | en_US |
| dc.title | STUDIES ON CALLUS INDUCTION POTENTIAL IN LOCAL PADDY VARIETIES Oryza sativa L. indica | en_US |
| dc.type | Thesis | en_US |