Development of an Enzyme Linked Immunosorbent Assay for Estimation of Buffalo Immunoglobulins Using Anti Bovine Light Chain Monoclonal Antibodies
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Date
2003
Journal Title
Journal ISSN
Volume Title
Publisher
Buffalo J.
Abstract
The monoclonal antibodies (Mabs) against bovine light chain cross-reacted with buffalo immunoglobulin (Ig) in radio immune assay. These Mabs were used as capture antibodies in an enzyme linked immunosorbent assay (ELISA) to capture all buffalo Ig isotypes in serum and colostrums. The captured Igs were detected using a commercial antibovine peroxidise conjugate. This test was compared to the conventional radial immune diffusion (RID) test using purified buffalo If from colostrums and antisera raised against it. Of the 61 samples of serum and colostrums analyzed using both these tests, the range of Ig concentrations in buffalo serum using RID and ELISA were respectively 7.44 – 39.10 mg/ml and 14.67 – 73.66 mg/ml (n=47). Similar concentration in colostrums was 22.32 – 90.77 by RID and 19.83 – 94.75 mg/ml by ELISA (n=14). The correlation coefficient of these concentrations together was 0.91. The capture ELISA was simple to perform, rapid and can be used to rapidly identify buffalo calves with ‘Failure of passive transfer’ to prevent neonatal calf mortality.
Description
Keywords
Monoclonal Antibodies, Enzyme-Linked Immunosobent Assay, Radial Immuno diffusion, Immunoglobulins