IN VITRO PRODUCTION OF DOUBLED HAPLOIDS FROM KRH-4 RICE HYBRID AND DEVELOPMENT OF STRATEGIES TO IDENTIFY HAPLOIDS AT EARLY DEVELOPMENTAL STAGES
| dc.contributor.advisor | MOHAN RAJU, B | |
| dc.contributor.author | CHAITANYA, GHALAGI | |
| dc.date.accessioned | 2023-01-03T12:11:08Z | |
| dc.date.available | 2023-01-03T12:11:08Z | |
| dc.date.issued | 2022-05-08 | |
| dc.description.abstract | In vitro androgenesis is an attractive biotechnological technique that provides rapid transformation of heterozygous hybrids into homozygous lines and thus significantly reducing the duration of breeding cycle. Although, it is recognized as a valuable tool, its application has been limited due to difficulties in induction of embryogenic calli, frequent regeneration of albino plants, low plant regeneration ability and poor acclimatization of in vitro grown plantlets in pot or field condition. The present study therefore aims at developing relatively a large number of doubled haploid (DH) lines from KRH-4 rice hybrid by optimizing the androgenic protocol, specifically to make indica rice anther culture easier and more efficient as well as successfully acclimating the in vitro plantlets under ex-vitro conditions. Accordingly, with new protocol, more number of DH lines were produced and with improvised hardening protocol, seedling mortality of in-vitro grown plants was reduced significantly. In addition, study also discovered phenotypic markers that distinguish haploids from doubled haploids at a very early growth stage enabling for commercial production of DH in rice similar to maize by addressing high cost of ploidy analysis and maintaining haploids until maturity. Thus, this finding establishes acute leaf apex’ shape as an accurate visual marker to rapidly identify haploid rice lines at an early developmental stage in a cost-effective manner with high accuracy like flow cytometry. Additionally, present study also assessed the stress response of selected haploids and DH rice lines under phenomics facility. The selected haploid lines observed to show lower growth and physiological response to moisture stress conditions than selected DH lines. Further, when characterized under aerobic condition, DH lines showed variation for a few growth and yield attributes and based on the yield, best DH lines were identified. These lines can form as potential lines for crop improvement or for release as varieties. | en_US |
| dc.identifier.other | Th-13276 | |
| dc.identifier.uri | https://krishikosh.egranth.ac.in/handle/1/5810191065 | |
| dc.keywords | VITRO PRODUCTION, RICE HYBRID | en_US |
| dc.language.iso | English | en_US |
| dc.pages | 175 | en_US |
| dc.publisher | University of Agricultural Sciences, Bangalore | en_US |
| dc.sub | Crop Physiology | en_US |
| dc.theme | RICE HYBRID AND DEVELOPMENT OF STRATEGIES TO IDENTIFY HAPLOIDS | en_US |
| dc.these.type | Ph.D | en_US |
| dc.title | IN VITRO PRODUCTION OF DOUBLED HAPLOIDS FROM KRH-4 RICE HYBRID AND DEVELOPMENT OF STRATEGIES TO IDENTIFY HAPLOIDS AT EARLY DEVELOPMENTAL STAGES | en_US |
| dc.type | Thesis | en_US |