Pathotyping of Newcastle disease virus by mean death time and TaqMan MGB probe real time PCR assay; a comparative study
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Date
2020-02
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Abstract
Newcastle disease (ND) is a standout amongst the most significant diseases of poultry throughout the
world. The disease is associated with serious respiratory, gastrointestinal, and neurological lesions in chicken.
The etiological agent of the disease is an Avian avulavirus 1, synonymously referred as Newcastle disease
virus (NDV). In vivo pathogenicity assaying is sensitive and specific pathotyping tools for detection and
identification of NDV used until the recent past. In this study twelve NDV isolates (Isolate numbers 463,
464, 475, 476, 122-17C, 122-17D, 122-17E, 128-17A, 128-17D, 137, 139, 141) available in the department
of Veterinary Microbiology, Madras Veterinary College (MVC), Chennai are subjected for pathotyping using
mean death time (MDT) in specific pathogen free (SPF) embryonated eggs and TaqMan minor groove binding
(MGB) probe real time PCR assay. Pathotyping based on the MDT revealed two NDV isolates (isolate no. 476
and 128-17D) as velogenic strains and remaining ten NDV isolates as lentogenic strains. Pathotyping based
on TaqMan MGB probe real time PCR assay revealed six NDV isolates (476, 128-17D, 463, 464, 475, 137)
as velogenic/mesosogenic strains and remaing six NDV isolates (122-17C, 122-17D, 122-17E 128-17A, 139,
141) as lentogenic strains. Four NDV isolates (463, 464, 475, 137) which are pathotyped as lentogenic strains
by MDT were pathotyped as velogenic/mesosogenic strains by real time PCR assay this shows the higher
sensitivity of TaqMan MGB probe real time PCR assay in pathotyping of NDV over conventional MDT.
Description
TNV_20thMVC_PP_Feb-2020_OP65
Keywords
Veterinary Science