IDENTIFICATION OF INTROGRESSED BACKCROSS LINES FOR LEAF RUST RESISTANCE IN BREAD WHEAT (Triticum aestivum L.) THROUGH MARKER ASSISTED SELECTION
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Date
2015-05
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University of Agricultural Sciences, Dharwad
Abstract
The present investigation was carried out to identify introgressed backcross
segregants carrying leaf rust resistance genes, Lr24 and Lr28 (from donor parent NIL
PBW343) into an elite, but susceptible bread wheat cultivar, DWR 162 background, using
marker assisted foreground and background selection.
Foreground selection for Lr24 and Lr28 was carried out using SCAR markers SCS719
and SCS1302 linked to Lr24 and SCAR marker SCS421 and SSR marker Xwmc313 linked to
Lr28. Background selection in BC2F2 population was carried out using forty two SSR markers
that were polymorphic between the parents DWR 162 and NIL PBW 343. Mean recurrent
parent genome (RPG) recovered was 80.3% with a range from 67.5-94.7%. Individual BC2F2
plants were observed for 9 DUS (Distinctiveness, Uniformity and Stability) characters to
identify plants which had recovered the characters of DWR 162.
Genetic variability studies in BC2F2 population indicated high PCV and GCV for grain
yield per plant (g) and high PCV and moderate GCV for number of productive tillers per plant,
moderate PCV and GCV for number of spikelets per spike. High heritability and GAM were
observed for number of tillers per plant. High heritability and moderate GAM was observed for
the traits plant height (cm), spike length and number of spikelets per spike. For leaf rust
infection, high PCV and GCV, moderate heritability and high GAM was recorded, suggesting
that there is high variability for this trait in BC2F2 population of the cross DWR 162 X NIL PBW
343. BC2F2, F3 and F4 generation plants with both Lr24 and Lr28 genes indicated superior
performance as compared to plants with either Lr24 or Lr28 leaf rust resistance genes.
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