Selection of Parents for Developing the Mapping Populations And Validation of Markers for Studying Pod Shattering in Mungbean (Vigna radiata)
| dc.contributor.advisor | Bhat, Sumangala | |
| dc.contributor.author | Nirmalbharathi M. | |
| dc.date.accessioned | 2019-09-23T11:10:51Z | |
| dc.date.available | 2019-09-23T11:10:51Z | |
| dc.date.issued | 2015-11 | |
| dc.description.abstract | Seventy six mungbean (Vigna radiata) genotypes were screened for pod shattering and related traits during summer 2014, kharif 2015 and summer 2015 under field condition. Also laboratory method such as incubation at room temperature and alternate wetting and drying were standardized and the genotypes showed significant variation for the traits studied. Summer season was found to be more suitable for pod shattering. The percentage of pod shattering ranged from 0- 54%. Based on the pod shattering percentage, genotypes were grouped into five classes according to the scale given by AVRDC, (1979). Using single marker analysis fourteen already identified SSRs markers were used to validate the germplam. Of which 3 markers were found to be significantly associated with pod shattering and five showed association with yield traits. One marker (CEDG245) showed significant association with pod shattering in field, incubation at room temperature, alternate wetting and drying with 3.48%, 12.00%, and 3.90% of R2 value respectively. Chinamung and GPM-19 showed high susceptibility to shattering and Pantmung-2, SML-108, NM-1 and COGG912 showed high resistance. The maximum pod shattering observed during summer 2015 was in Chinamung (52%) and GPM-19 (54%) where as Pantmung-2, SML-108 and NM-1 did not show any shattering. These resistant and susceptible genotypes were screened with 34 SSR markers and total 12 polymorphic SSR makers were identified between Chinamung and Pantmung-2 and nine were between Chinamung and SML-108. Molecular diversity analysis showed resistant and susceptible parents come under different clusters. Also PIC value of 34 markers ranged from 0 (GMES4232 and CEDG012) to 0.89 (X-20 and X-21) with an average of 0.41. Two sets of crosses were made to develop the mapping population viz., Chinamung x Pantmung-2 and Chinamung x SML108. Using polymorphic markers true hybrid nature was confirmed in both the crosses and F2 seeds were collected. | en_US |
| dc.identifier.uri | http://krishikosh.egranth.ac.in/handle/1/5810129038 | |
| dc.keywords | Selection of Parents for Developing the Mapping Populations And Validation of Markers for Studying Pod Shattering in Mungbean (Vigna radiata) | en_US |
| dc.language.iso | en | en_US |
| dc.pages | 102p | en_US |
| dc.publisher | University of Agricultural Science, Dharwad | en_US |
| dc.research.problem | Selection of Parents for Developing the Mapping Populations And Validation of Markers for Studying Pod Shattering in Mungbean (Vigna radiata) | en_US |
| dc.sub | Biotechnology | en_US |
| dc.subject | null | en_US |
| dc.theme | Selection of Parents for Developing the Mapping Populations And Validation of Markers for Studying Pod Shattering in Mungbean (Vigna radiata) | en_US |
| dc.these.type | M.Sc | en_US |
| dc.title | Selection of Parents for Developing the Mapping Populations And Validation of Markers for Studying Pod Shattering in Mungbean (Vigna radiata) | en_US |
| dc.type | Thesis | en_US |