IDENTIFICATION OF DIAGNOSTIC MOLECULAR MARKERS FOR GENETIC PURITY TESTING IN COWPEA [Vigna unguiculata (L.) Walp.]
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Date
2018-09-08
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University of Agricultural Sciences, Bengaluru
Abstract
Genetic purity is one of the important quality criteria required for successful seed
production and seed certification. Hence, the experiment was set to evaluate the
usefulness of SSR markers to determine genetic purity of commercial seed lots of
cowpea, which are present in the seed chain of Karnataka. Out of 55 SSR markers
studied, 10 SSR markers showed polymorphism for each of the 7 varieties, which
revealed 25 alleles at 10 loci with an average of 2.5 alleles per locus. The SSR 6292
showed the highest number of alleles with the PIC value of 0.71. PKB-4 cultivar was
found to be most divergent from dendrogram constructed by UPGMA clustering. The
genetic purity of the seed lots was tested by following the 20 × 20 matrix, where the
purity levels recorded cent per cent for all the seed lots except for KBC-2, IT-38956-1
and C-152 varieties, for which the purity levels are 98%, 96% and 97%, respectively.
This analysis revealed that SSR marker is a potential tool for fingerprinting of varieties as
well as for the genetic purity testing of the seed lots. Thus, it is helpful for delivering pure
seed lots to the farmers.
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