CHARACTERIZATION OF INDIAN ISOLATES OF Clostridium Chauvoei BY 23S rDNA SEQUENCE ANALYSIS
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Date
2014-01
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Abstract
Black quarter is an important bacterial disease affecting cattle and sheep, caused by Clostridium chauvoei, a gram positive, sporulating anaerobic bacteria. The conventional methods for diagnosing the disease, including cultural and biochemical methods, have drawbacks such as lack of specificity, tediousness and require pure culture. A 16S-23S rDNA gene specific PCR was used to identify the C. chauvoei from the muscles samples of the affected cattle. It was followed by 23S rDNA gene sequence analysis. The sequence analysis revealed that the two Indian isolates of C. chauvoei have 97.7-98.0% similarity with the C. chauvoei strain ATCC 10092 and C. chauvoei strain BS108-01, confirming that the isolates were C. chauvoei. Phylogenetic analysis had shown that Indian isolates clustered with C. chauvoei ATCC 10092 and BS108-01 strains. A PCR assay using 16S-23S rDNA spacer gene primers followed by 23S rRNA gene sequence analysis can be used to identify and confirm the C. chauvoei isolates.
Description
TNV_IJCMIID_2014_35(1)18-21
Keywords
Veterinary Science