DEVELOPMENT OF HOUSEHOLD WASTES MEDIA FOR MASS PRODUCTION OF MICROBIAL INOCULANTS
| dc.contributor.advisor | Kumari, Geeta | |
| dc.contributor.author | KUNDU, PUJA | |
| dc.date.accessioned | 2023-01-23T10:40:00Z | |
| dc.date.available | 2023-01-23T10:40:00Z | |
| dc.date.issued | 2022 | |
| dc.description.abstract | Wastes generated in our house contain a large amount of valuable nutrients; can be used as substitute to develop alternate media for microbial inoculants. In this study, different household vegetable and fruit wastes were collected from different places. Nutrient content (N, P, K, Na, Ca, Mg, Fe, Mn), pH, carbohydrate, and protein content of these wastes were analyzed. Out of 10 wastes collected, 6 wastes (moringa leaves, pumpkin seeds, banana peel, beet peel, palak root and stalk, sugarcane bagasse) were selected based on their nutrient content (nitrogen, phosphorus, potassium, calcium, magnesium, sodium, iron, manganese, carbohydrate, and protein). Palak root and stalk was found to be rich in calcium, magnesium, sodium, iron, and manganese. While, pumpkin seeds exhibited high amount of phosphorus. However, banana peels, moringa leaves and sugarcane bagasse illustrated highest content of potassium, protein and carbohydrates, respectively. 18 household waste based liquid formulations replacing commercially available media (YEMA, NA, and KB) were designed with different combinations [commercially available media + household waste + protectants + microbial inoculants (Bacillus sp. (BS5), Pseudomonas sp. (PS2), and Rhizobium sp (RZM2)]. Viability of newly developed household waste based liquid formulation was examined at different storage temperatures (40C, 280C and 400C) at monthly intervals up to 6 months of storage period. After six months of storage, the formulations stored at 4°C exhibited the highest cell count, stable pH, and low suspensibility followed by formulations stored at 28°C and 40°C. Out of 18 newly developed household waste based liquid formulations; the formulation FYEMA4 depicted highest cell count, stable pH and lowest suspensibility followed by FNA4 and then FKB4 at 4°C, 28°C and 40°C till 6th month. All developed formulations retained nutrient solubilization characteristics (phosphorus solubilization, potassium solubilisation, siderophore production efficiency and zinc solubilisation). Liquid formulation namely FYEMA4 showed highest nutrient solubilisation potential even after longer storage period (at sixth month) followed by FNA4 and FKB4. This study revealed that household waste contain good amount of nutrients. Therefore, these wastes could be used as alternative nutrient source to design liquid media based formulation for microbial cultivation. | en_US |
| dc.identifier.other | M/Micro/045/2020-21 | |
| dc.identifier.uri | https://krishikosh.egranth.ac.in/handle/1/5810192690 | |
| dc.keywords | Development, household wastes media, mass production, microbial inoculants, Agricultural Microbiology. | en_US |
| dc.language.iso | English | en_US |
| dc.pages | 79 + i-xix (Bibliography) | en_US |
| dc.publisher | DRPCAU, PUSA | en_US |
| dc.sub | Microbiology | en_US |
| dc.theme | Development of household wastes media for mass production of microbial inoculants | en_US |
| dc.these.type | M.Sc | en_US |
| dc.title | DEVELOPMENT OF HOUSEHOLD WASTES MEDIA FOR MASS PRODUCTION OF MICROBIAL INOCULANTS | en_US |
| dc.type | Thesis | en_US |