DEVELOPMENT OF HOUSEHOLD WASTES MEDIA FOR MASS PRODUCTION OF MICROBIAL INOCULANTS
Loading...
Date
2022
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
DRPCAU, PUSA
Abstract
Wastes generated in our house contain a large amount of valuable nutrients;
can be used as substitute to develop alternate media for microbial inoculants.
In this study, different household vegetable and fruit wastes were collected
from different places. Nutrient content (N, P, K, Na, Ca, Mg, Fe, Mn), pH,
carbohydrate, and protein content of these wastes were analyzed. Out of 10 wastes
collected, 6 wastes (moringa leaves, pumpkin seeds, banana peel, beet peel, palak root
and stalk, sugarcane bagasse) were selected based on their nutrient content (nitrogen,
phosphorus, potassium, calcium, magnesium, sodium, iron, manganese, carbohydrate,
and protein). Palak root and stalk was found to be rich in calcium, magnesium,
sodium, iron, and manganese. While, pumpkin seeds exhibited high amount of
phosphorus. However, banana peels, moringa leaves and sugarcane bagasse illustrated
highest content of potassium, protein and carbohydrates, respectively. 18 household
waste based liquid formulations replacing commercially available media (YEMA,
NA, and KB) were designed with different combinations [commercially available
media + household waste + protectants + microbial inoculants (Bacillus sp.
(BS5), Pseudomonas sp. (PS2), and Rhizobium sp (RZM2)]. Viability of newly
developed household waste based liquid formulation was examined at different
storage temperatures (40C, 280C and 400C) at monthly intervals up to 6 months of
storage period.
After six months of storage, the formulations stored at 4°C exhibited the
highest cell count, stable pH, and low suspensibility followed by formulations stored
at 28°C and 40°C.
Out of 18 newly developed household waste based liquid formulations; the
formulation FYEMA4 depicted highest cell count, stable pH and lowest suspensibility
followed by FNA4 and then FKB4 at 4°C, 28°C and 40°C till 6th month. All developed
formulations retained nutrient solubilization characteristics (phosphorus
solubilization, potassium solubilisation, siderophore production efficiency and zinc
solubilisation). Liquid formulation namely FYEMA4 showed highest nutrient
solubilisation potential even after longer storage period (at sixth month) followed by
FNA4 and FKB4.
This study revealed that household waste contain good amount of nutrients.
Therefore, these wastes could be used as alternative nutrient source to design liquid
media based formulation for microbial cultivation.