ADVENTITIOUS SHOOT REGENERATION FROM LEAVES OF APPLE ROOTSTOCK MERTON I.793
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Date
2014
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ABSTRACT
The present studies were aimed to develop an efficient and reproducible method for shoot regeneration from leaf explants of apple rootstock Merton I793.The influence of two cytokinins BA and TDZ with three auxins IAA, IBA and NAA in different combinations were evaluated for shoot induction using light and dark incubation. Direct and healthy shoots were obtained from leaves on MS medium with BA-NAA and BA-IAA combinations in light incubation and 100% regeneration was achieved on medium with 4 mg/l BA and 1 mg/l NAA . Both direct and indirect shoot regeneration was obtained on TDZ-NAA and TDZIBA. Maximum shoot regeneration was promoted on 0.8&0.6 mg/l TDZ with 1 mg/l NAA in light. Dark treated leaves showed low rates of regeneration with TDZ combinations. RAPD analysis of regenerants using 5 primers revealed that the shoots which originated directly from leaves were similar to mother plant due to monomorphism, while shoots originated through intervening callus revealed 61.2% polymorphism.