Influence of seed priming with methyl-Jasmonic acid on expression of defense related transcription factors and tolerance to Alternaria blight disease in Brassica juncea plants

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dc.contributor.advisorPandey, Dinesh
dc.contributor.authorKumar, Piyush
dc.date.accessioned2024-11-29T12:03:17Z
dc.date.available2024-11-29T12:03:17Z
dc.date.issued2023-07-01
dc.description.abstractRapeseed mustard ( Brassicajuncea ), an important oilseed crop, faces significant challenges due to diseases such as Alternaria blight, which cause substantial yield losses. This study aimed to investigate the effects of Methyl Jasmonic Acid (MeJA) seed priming on Alternaria blight disease and role of WRKY, MYC, and NAC transcription factors in Brassica juncea in enhancing tolerance against Alternaria blight disease. Jasmonic acid is of significant importance as a signal molecule in triggering defence responses when plants enco unter wounds, herbivore assaults, or pathogen infiltrations. Seeds were treated with different concentrations (1mM, 0.1mM, and 0.01mM) of MeJA for various durations (2 hours, 4 hours, and 6 hours). The viability and seedling growth of treated seeds were ev aluated alongside non primed/control plants. The lower concentration of JA (0.1 mM and 0.01mM) enhanced seed germination percentage with increase in shoot and root length. Following seed priming, plants were developed and subjected to Alternaria blight dis ease by spraying with Alternaria brassicae spores, and disease indices were recorded at different time points.The results indicated that MeJA seed priming significantly inhibited the development of Alternaria blight disease. Treated plants exhibited lower disease symptoms compared to the control plants. Depending on concentration and duration disease percentages ranged from 22.4% to 34% in MeJA treated plants , whereass in non primed/control plant disease percentages was observed 43% at 21 DPI. MeJA priming enhanced the expression of WRKY, MYC, and NAC transcription factors, potentially contributing to disease tolerance. RT PCR based analysis confirmed the 3 to 4 fold increase in expression levels of these genes in MeJA primed plants, as compared to the cont rol non primed plants. In conclusion, MeJA seed priming induced the expression of WRKY, MYC, and NAC transcription factors, enhancing tolerance against Alternaria blight disease in Brassicajuncea . These findings highlight the potential of MeJA seed priming as a management technique to improve Alternaria blight disease resistance in economically important Brassica crops.
dc.identifier.citationAcademic Research
dc.identifier.urihttps://krishikosh.egranth.ac.in/handle/1/5810216746
dc.keywordsmethyl-Jasmonic acid
dc.keywordsseed priming
dc.language.isoEnglish
dc.pages79 p. p.
dc.publisherG.B. Pant University of Agriculture and Technology, Pantnagar-263145
dc.relation.ispartofseries11129
dc.subBiotechnology
dc.themeAcademic Research
dc.these.typeM.Sc
dc.titleInfluence of seed priming with methyl-Jasmonic acid on expression of defense related transcription factors and tolerance to Alternaria blight disease in Brassica juncea plants
dc.typeThesis
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