EVALUATION OF N-ACETYL CYSTEINE AGAINST EXPERIMENTAL TOXICITY DUE TO CYCLOPHOSPHAMIDE IN RATS
Loading...
Files
Date
2008-12
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
SRI VENKATESWARA VETERINARY UNIVERSITY TIRUPATI - 517 502. (A.P.) INDIA
Abstract
ABSTRACT:
The present study was aimed to evaluate the protective effect of N-acetyl cysteine (NAC) in cyclophosphamide (CYP)-induced toxicity. Female albino rats of Wistar Kyoto strain were divided into four groups and treated as follows: Group 1 served as basal diet control, groups 2, 3 and 4 received CYP on 1st, 3rd, 5th and 7th day. Group 2 served as toxic control. Group 3 was treated with NAC from 1st to14th day while group 4 received NAC from 7th to 14th day. SOD and catalase, biomarkers of cardiac and renal damage were recorded at the end of 1st and 2nd wk. At the end of 2nd wk estimation of TBARS, protein carbonyls, GSH of heart and kidney, Na+ K+ ATPase of heart, and histopathology of heart and kidney were done.
The levels of CPK, LDH, troponins, BUN, serum creatinine, TBARS and protein carbonyls were significantly (P < 0.05) increased, while the activity of catalase, SOD and Na+-K+ ATPase, and concentration of GSH were significantly (P < 0.05) decreased in CYP toxic group. NAC treatment for 14 days produced improvement on heart, while 7 days treatment has no recognizable effect. NAC could not produce any protective effect on kidney damage induced by CYP. Histological abnormalities were observed in CYP control group at the end of 1st and 2nd wk. Group 1 did not reveal any abnormalities in histopathology. Heart tissue of NAC treated rats showed lesions of mild intensity, while kidney lesions were unaffected. From this study, it is concluded that N-acetyl cysteine is protective against CYP-induced toxic effects in heart but not in kidney.
Description
THESES
Keywords
null