Comparison of Different DNA Extraction Procedures for the Detection of Sheep Pox Virus by PCR
| dc.contributor.author | Vadivoo, V.S. | |
| dc.contributor.author | Ramesh, A. | |
| dc.contributor.author | Babu, S. Suresh | |
| dc.contributor.author | Saravanabava, K. | |
| dc.contributor.author | TANUVAS | |
| dc.date.accessioned | 2016-07-13T12:45:53Z | |
| dc.date.available | 2016-07-13T12:45:53Z | |
| dc.date.issued | 2009-08 | |
| dc.description.abstract | Scab samples obtained from suspected outbreaks of sheep pox infection were subjected to PCR assay using the Ins-1.1/Ins-1.1' primer pair specific for sheep pox virus. Three known positive clinical samples were subjected to three different procedures of DNA extraction. The DNA template obtained by boiling method showed smearing on the agarose gel electrophoresis. The modified phenol-chloroform method of DNA extraction yielded good quantity of DNA than the commercial DNA extraction kit. The purity of the extracted DNA by both the modified phenol-chloroform method and commercial DNA extraction kit method was found to be almost equal. Thus the modified phenol-chloroform method although time consuming was found to be simple and inexpensive and could be employed for large number of samples. Whereas the expensive commercial DNA extraction kit method could be employed when results are required within a day. | en_US |
| dc.identifier.uri | http://krishikosh.egranth.ac.in/handle/1/68687 | |
| dc.language.iso | en | en_US |
| dc.publisher | Indian Veterinary Journal | en_US |
| dc.title | Comparison of Different DNA Extraction Procedures for the Detection of Sheep Pox Virus by PCR | en_US |
| dc.title.alternative | IVJ_V86_I08_Pg767-769 | en_US |
| dc.type | Article | en_US |