CHARACTERIZATION OF PLANT GROWTH PROMOTING RHIZOBACTERIA AND EVALUATION OF THEIR SYNERGISTIC POTENTIAL WITH Rhizobium IN LENTIL (Lens culinaris Medikus

dc.contributor.advisorVeena, Khanna
dc.contributor.authorPreeti Saini
dc.date.accessioned2017-06-14T09:45:44Z
dc.date.available2017-06-14T09:45:44Z
dc.date.issued2011
dc.description.abstractForty-eight rhizobacterial isolates obtained from lentil rhizospheric soils from 34 locations were characterized and found belonging to genera Bacillus (23), Pseudomonas (23) and Serratia (2). The isolates were evaluated for their plant growth and antagonistic traits. Fifteen of the isolates produced siderophore as evidenced on Chrome-azurol-S agar, maximum catechol-type siderophore being produced by B-40 (97μg/ml), while maximum hydroxamate-type siderophore by B-20 (129.5μg/ml). Phosphorus solubilization was recorded with 39.5% isolates and the relative efficiency of P-solubilized in liquid medium ranged from 3.5-44mg/100ml. All these isolates produced the phytostimulator Indole-acetic-acid which ranged from 3-106.1μg/ml. Nine of the isolates were able to utilize ACC as the sole N-source, their ACC-metabolizing rate was measured as optical density (OD496) ranged from 0.54-1.39. The antagonistic ability against Fusarium oxysporum ranged from 3.3–16.6%, maximum being recorded with P-1 (16.6%), followed by B-36 (13.3%) and B-40 (3.3%). Similar trend was recorded in liquid medium. On the basis of their PGP traits B-40 and P-1 were selected for field studies. These isolates showed compatibility with Rhizobium leguminosarum under axenic conditions and also promoted root/shoot growth in lentil seedlings. The effect of selected PGPR and Rhizobium sp. along with check strain KBB-133 was studied on lentil crops in the field. The results showed that maximum increase in symbiotic parameters was observed in case of dual inoculations of PGPRs with Rhizobium. The grain yield with dual inoculations with B-40 (1703 kg/ha) and P-1 (1679 kg/ha) was statistically on par to dual inoculation with KBB-133 (1698 kg/ha).en_US
dc.identifier.urihttp://krishikosh.egranth.ac.in/handle/1/5810020146
dc.keywordsKey Words: Lentil, PGPR, Rhizobium, Synergismen_US
dc.language.isoenen_US
dc.publisherPAU Ludhianaen_US
dc.subMicrobiologyen_US
dc.subjectnullen_US
dc.themeRHIZOBACTERIAen_US
dc.these.typeM.Scen_US
dc.titleCHARACTERIZATION OF PLANT GROWTH PROMOTING RHIZOBACTERIA AND EVALUATION OF THEIR SYNERGISTIC POTENTIAL WITH Rhizobium IN LENTIL (Lens culinaris Medikusen_US
dc.typeThesisen_US
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