CHARACTERIZATION OF PLANT GROWTH PROMOTING RHIZOBACTERIA AND EVALUATION OF THEIR SYNERGISTIC POTENTIAL WITH Rhizobium IN LENTIL (Lens culinaris Medikus
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Date
2011
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PAU Ludhiana
Abstract
Forty-eight rhizobacterial isolates obtained from lentil rhizospheric soils from 34
locations were characterized and found belonging to genera Bacillus (23), Pseudomonas (23)
and Serratia (2). The isolates were evaluated for their plant growth and antagonistic traits.
Fifteen of the isolates produced siderophore as evidenced on Chrome-azurol-S agar,
maximum catechol-type siderophore being produced by B-40 (97μg/ml), while maximum
hydroxamate-type siderophore by B-20 (129.5μg/ml). Phosphorus solubilization was recorded
with 39.5% isolates and the relative efficiency of P-solubilized in liquid medium ranged from
3.5-44mg/100ml. All these isolates produced the phytostimulator Indole-acetic-acid which
ranged from 3-106.1μg/ml. Nine of the isolates were able to utilize ACC as the sole N-source,
their ACC-metabolizing rate was measured as optical density (OD496) ranged from 0.54-1.39.
The antagonistic ability against Fusarium oxysporum ranged from 3.3–16.6%, maximum
being recorded with P-1 (16.6%), followed by B-36 (13.3%) and B-40 (3.3%). Similar trend
was recorded in liquid medium. On the basis of their PGP traits B-40 and P-1 were selected
for field studies. These isolates showed compatibility with Rhizobium leguminosarum under
axenic conditions and also promoted root/shoot growth in lentil seedlings. The effect of
selected PGPR and Rhizobium sp. along with check strain KBB-133 was studied on lentil
crops in the field. The results showed that maximum increase in symbiotic parameters was
observed in case of dual inoculations of PGPRs with Rhizobium. The grain yield with dual
inoculations with B-40 (1703 kg/ha) and P-1 (1679 kg/ha) was statistically on par to dual
inoculation with KBB-133 (1698 kg/ha).
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