Evaluation of different okra (Abelmoschus esculentus L. moench) genotypes for nutritional and antinutritional components

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dc.contributor.advisorSangha, Manjeet Kaur
dc.contributor.authorParminder Kumar
dc.date.accessioned2018-10-14T05:01:14Z
dc.date.available2018-10-14T05:01:14Z
dc.date.issued2018
dc.description.abstractThe present study evaluated green pods and mature seeds of 166 okra genotypes for phenotypic trait, nutritional and antinutritional component variation. Phenotypic variation was observed for 32 qualitative and quatitative traits. Pearson product correlation correlated fruits/plant positively and significantly with number of internodes and plant height. Fruit total carbohydrates varied from 39.11-82.1% (DW basis). Total soluble sugars (TSS), reducing sugars, starch, sucrose and mucilage ranged from 3.39-20.98%, 0.67-1.86%, 30.26-65.6%, 11.34-16.9% and 3.01-8.14% respectively in pods. Total carbohydrates, TSS and reducing sugars in seeds varied from 15.79- 38.99%, 3.67-9.78% and 1.39-4.35% respectively. Crude protein, TSP and free amino acids in pods varied from 13.3%- 33.8%, 8.17- 21.0% and 0.62- 5.07% respectively. In seeds they ranged from 14.49- 37.83%, 6.61- 25.41%, and 0.44- 1.39% respectively. Lysine, methionine and tryptophan varied from 0.54- 1.29%, 0.06- 1.44 (mg/g) and 0.62- 2.23% respectively. Crude fiber, ADF and NDF in pods ranged from 5.21-8.98%, 8.29-14.92 and 12.73-24.35%. The seed oil content varied from 2.33-18.01%. 16:0, 18:0, 18:1 and 18:2 registered variations of 24.52- 45.64%, 0.01- 6.81%, 21.62- 39.63% and 23.95- 48.78% respectively. In pods total phenols, odihydroxy phenols and flavonols ranged from 385.71-939.421mg/100g, 20.09-79.451mg/100g and 11.43-98.1mg/100g DW. Whereas phenols and flavonols in seeds ranged from 3.18-7.32 mg/100g and 12.17-39.35 mg/100g. Chlorophyll a, b and carotenoids in fruits varied from 1.38- 11.15, 0.42-5.57 and 0.24-1.86mg/g respectively. Vitamin C and E ranged from 18.7-33.90.59 mg/g and 0.16-0.59 mg/g respectively. FRAP (2.28 mg/g AAE) and DPPH (89.89% inhibition) activity was maximum in Punjab Padmini and IC0506229 respectively. The carotenoids and tocopherols in seeds varied from 0.36-1.43mg/100g and 298.8-985.3ppm respectively. Phytate, saponins, tannins and oxalate in pods ranged from 1.7- 2.94%, 0.41- 1.32%, 10.36- 99.76% and 0.41- 0.95% respectively. In seeds the range was 0.21-0.75%, 1.72-3.44%, 11.67-38.47% and 0.27-1.62% respectively. Principal components analysis showed total variation of 77.17% and 82.49% in pods and mature seeds respectively.Genotypes (IC0043741, IC0506056, IC0506096, IC0510679, POL-311, POL-325) with high total protein content were subjected to SDS-PAGE. Globulins constituted maximum portion (30.53%) of seed protein followed by albumins (17.87%), glutelins (7.86%) and prolamines (4.43%). SDS pattern of the glutelins showed maximum variability. Fifteen elite genotypes (with high levels of quality parameters and low levels of antinutritionals) were selected out which can be exploited for okra improvement programmes and industrial and pharmaceutical applications.en_US
dc.identifier.urihttp://krishikosh.egranth.ac.in/handle/1/5810081053
dc.keywordsOkra, phenotypic traits, nutrients, antioxidants, antinutrients, seed storage proteins, Abelmoschus esculentus L. moenchen_US
dc.language.isoenen_US
dc.pages136en_US
dc.publisherPunjab Agricultural University, Ludhianaen_US
dc.research.problemEvaluation of different okra (Abelmoschus esculentus L. moench) genotypes for nutritional and antinutritional componentsen_US
dc.subBiochemistryen_US
dc.subjectnullen_US
dc.themeEvaluation of different okra (Abelmoschus esculentus L. moench) genotypes for nutritional and antinutritional componentsen_US
dc.these.typePh.Den_US
dc.titleEvaluation of different okra (Abelmoschus esculentus L. moench) genotypes for nutritional and antinutritional componentsen_US
dc.typeThesisen_US
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