COMPARATIVE EVALUATION OF WOUND HEALING PROPERTIES OF Cinnamomum verum J.S.Presl MEDIATED PHYTOGENIC AND CHEMICALLY DERIVED NANOSILVER IN WISTAR RATS
| dc.contributor.advisor | ADILAXMAMMA, K (Major) | |
| dc.contributor.advisor | ALPHARAJ, M | |
| dc.contributor.advisor | ESWARA PRASAD, P | |
| dc.contributor.author | SREEVANI, P | |
| dc.date.accessioned | 2017-01-03T15:39:37Z | |
| dc.date.available | 2017-01-03T15:39:37Z | |
| dc.date.issued | 2012-10 | |
| dc.description | THESES | en_US |
| dc.description.abstract | ABSTRACT : An experimental study was conducted on wistar albino rats to evaluate and compare the wound healing properties of Cinnamomum verum mediated phytogenic and chemically derived (citrate coated) nanosilver in excision, incision and dead space wound models. The nanoparticles were synthesized and characterized using UV-Visible spectrophotometer, SEM, DLS technique and FT-IR analysis and were also evaluated for anti-bacterial activity and cytotoxic effects. A total of 90 rats (both sexes) were divided into five groups of 18 rats each and the wound healing activity of topical application of the compounds was evaluated in three different wound models viz., excision, incision and dead space. Group 1 was treated with normal saline, group 2 received Povidone iodine (1%), group 3 received citrate coated silver nanoparticles (NS) (170 μg per dressing), group 4 received C. verum bark aqueous extract (CE) (100 mg per dressing) and group 5 received C. verum bark extract mediated silver nanoparticles (CENS) (85 μg per dressing). In excision wound model, percentage wound contraction and period of epithelialization were recorded. On Day 10 of post wound, blood was collected from orbital sinus in EDTA coated tubes and plasma was separated for the estimation of Vitamin C, TBARS and total proteins. A small piece of granulation tissue along with a 5 mm margin of normal skin was excised around the wound edges and processed for histopathological examination. In incision wound model, the wound breaking strength was measured on 10th post wound day. In dead space wound model, the granulation tissue collected on Day 10 of post wound was used for the estimation of hydroxyproline, hexosamine, total protein, TBARS, superoxide dismutase, catalase, reduced glutathione and ascorbic acid. UV-Vis absorption spectra of NS and CENS showed absorption maxima between 380 to 450 nm and 390 to 550 nm, respectively. SEM micrograph of NS showed an agglomerated morphology with size ranging from 374 nm to 718 nm whereas CENS particles were grain in shape and monodispersive with size ranging from 100 nm to 1μm. The particle size as measured by the DLS technique of NS was 78.8 nm whereas that of CENS was 126.6 nm. The results of FT-IR analysis indicated the involvement of hydroxyl and primary amine functional groups in the synthesis of NS and hydroxyl, C-H, primary amines and secondary amides functional groups in the synthesis of CENS, respectively. The results of in vitro studies indicated that CENS showed better anti-bacterial properties against Staphylococcus aureus as evidenced by the highest zone of inhibition (18.67 mm) and lowest MIC value (7.09 μg/ml). Cytotoxicity studies revealed that CENS showed more than 90 % viability at the doses studied i.e., 2-85 ppm while NS exhibited toxicity from 10 ppm onwards in mouse spleenocytes. In excision wound model, CENS showed significantly higher percentage of wound contraction (97.9 %) and shortest period (19.8 days) of epithelialization compared to wound control group. Further, complete re-growth of hair and normal epithelium was restored in the mentioned period in CENS group. The lipid peroxidation was significantly lowered (p<0.05) and level of total protein was significantly (p<0.05) elevated in all treatment groups compared to control with CENS group showing the highest significant effect. The levels of vitamin C were significantly (p<0.05) elevated in plasma in both CE and CENS groups. Histopathological examination of CENS group showed mild cellular infiltration with angiogenesis and more fibroblast proliferation when compared to control. In the incision wound model, CE and CENS groups showed significantly (p<0.05) higher wound breaking strength compared to other groups. In dead space wound model, there was a significant (p<0.05) increase in hydroxyproline, hexosamine, total protein content, Vit C, catalase activity and decreased lipid peroxide levels in the granulation tissue of CENS group compared to other treatment groups. Thus in conclusion, C. verum bark aqueous extract mediated silver nanoparticles showed better wound healing properties compared to citrate coated silver nanoparticles, povidone iodine and aqueous extract of C. verum with complete epithelialization and normal re-growth of hair. | en_US |
| dc.description.sponsorship | DEPARTMENT OF PHARMACOLOGY & TOXICOLOGY , COLLEGE OF VETERINARY SCIENCE, TIRUPATI – 517 501 (A.P.) INDIA | en_US |
| dc.identifier.uri | http://krishikosh.egranth.ac.in/handle/1/94225 | |
| dc.language.iso | en | en_US |
| dc.publisher | Sri Venkateswara Veterinary University, TIRUPATI – 517 502,A.P | en_US |
| dc.sub | Veterinary Pharmacology | en_US |
| dc.subject | RATS ; WOUND HEALING; Cinnamomum verum ; NANOSILVER | en_US |
| dc.title | COMPARATIVE EVALUATION OF WOUND HEALING PROPERTIES OF Cinnamomum verum J.S.Presl MEDIATED PHYTOGENIC AND CHEMICALLY DERIVED NANOSILVER IN WISTAR RATS | en_US |
| dc.title.alternative | MVSC;CVSc;TPTY; Acc No:T1462 | en_US |
| dc.type | Thesis | en_US |