Phenotypic and molecular analysis for morpho-physiological traits and yellow rust in wheat progenies derived from the cross between WH711/WH542
Loading...
Date
2019-12
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
CCSHAU, Hisar
Abstract
The present investigation entitled “Phenotypic and molecular analysis for morpho-physiological traits and yellow rust in wheat progenies derived from the cross between WH711 / WH542” The experiments were conducted in the Department of Genetics and Plant Breeding, CCS Haryana Agricultural University (CCSHAU), Hisar, India during two successive years of Rabi seasons 2014-15 and 2015-16 with objective to determine variability, correlation path analysis and genetic divergence. Characterization of 238 progenies along with parent was done on the basis of 12 morpho-physiological traits which exhibited sufficient variation in all the traits. The differences between GCV and PCV values were less which showed little influence of environment. The correlation results revealed that plant height, number of tillers per plant, spike length, number of grains per spike, grain length, 1000 grain weight, biological yield per plant and harvest index expressed significant positive correlation with grain yield per plant across the generations and can be used for improving grain yield per plant. Path analysis of both generations revealed that biological yield per plant and harvest index had high direct effect on grain yield per plant while traits viz., number of tillers per plant, plant height and spike length contributed to grain yield per plant through biological yield per plant and harvest index. Based on D2 values, the progenies were grouped into 7 clusters in F3 and 8 clusters in F4 generation. The progenies included in clusters III, V and VI in F3 and V, VII and VIII in F4 generation were identified as divergent as well as having higher mean value for important yield component traits. The traits viz., grain breadth, number of grains per spike, spike weight, number of tillers per plant and 1000 grain weight contributed more than 60% towards total variability. Out of 438 SSR primers pairs (including Yr specific primers) used to characterize the parents for Yr gene and agronomic traits. Among 217 SSRs gave amplification. Out of these 217, SSR 145 markers were monomorphic and 72 SSR markers (including twelve Yr specific markers) were found polymorphic on parents. It was confirmed by NTSYS-PC UPGMA cluster tree and dendrogram that two parents were most genetically diverse and the progeny lines were interspersed between the two parents for yellow rust in both the generations. Single marker analysis indicated that markers Xgwm249 and Xgwm297 were linked with yellow rust resistance in F4 generation.