A study on the repellent and antifertility potential of neem seed extract against female Bandicota bengalensis
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Date
2018
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PUNJAB AGRICULTURAL UNIVERSITY,LUDHIANA
Abstract
Fifteen different doses of Neem seed alcoholic (NSA) extract (1, 1.5, 2.0, 2.5, 3.0, 3.5, 4.0, 4.5, 5.0, 5.5, 6.0, 6.5, 7.5, 8.0 and 9.0 ml out of 25ml stock NSA) were tested under laboratory condition to determine the effective dose with repellent effect. Results revealed antifeedant/ secondary repellent effect with all the doses and non-significant difference in antifeedant index (AI) among doses. Therefore for further studies, only three different doses (1.5, 7.5 and 9.0 ml) were selected. Testing of existence of primary repellent effect revealed that rats avoided all the treated baits for first five hours after exposure. After that rats developed habituation for primary/olfactory repellent effect and started eating treated baits which might had caused gastrointestinal malaise in rats resulting in development of secondary repellent effect among rats. However rats were not habituated for secondary repellent effect with all the selected doses of NSA extract upto experimental period of 30 days. There was also a significant increase in AI during treatment period Formulations F1 and F2 were tested under simulated field condition. Results revealed potential of F2 as repellent in preventing rodent damage for considerably long time as compared to F1. To use the antifertility property of neem seed, neem seed alcoholic hexane (NSAH) extract and its formulations as baits (F3–F7) were developed and fed to rats. Consumption of F7 bait was maximum. Results revealed potential of NSAH extract and its F7 bait in reducing reproductive potential of rats. However there is a need to increase the acceptance and application rate of F7 bait under field conditions.
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