STUDIES ON GENETIC DIVERSITY AND SEX LINKED MARKERS IN BETELVINE (Piper betle L.)
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Date
12-08-11
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University of Agricultural Sciences GKVK, Bangalore
Abstract
Betelvine is one of the heritage crops of India. In spite of being an
important cash crop very limited research has been carried out in this
crop. Molecular marker assisted investigations were undertaken to study
the genetic diversity of betelvine accessions and Piper species and to
identify of sex linked markers in P. betel. Genetic diversity analysis was
carried out by using Inter Simple Sequence Repeats (ISSR) markers in 38
accessions of betelvine and one accession each of P. colubrinum and P.
hamiltoni. Out of 60 ISSR primers tested, 15 were selected based on high
and consistent polymorphism. The 15 ISSR primers generated a total 82
bands of which 72 were polymorphic. The different band statistics and
efficiency parameters showed that the primers viz; UBC-822, 825, 826,
863 and ISSR-1, ISSR-15 were more efficient and UBC-828 the least
efficient primer to study the genetic diversity. Studies UPGMA
dendrogram and PCA plot revealed P. colubrinum to be the most distant
of the three species. The Andaman accessions of betelvine clustered
based on geographical origin and shared 70% similarity. Gender based
clustering was also observed in the betelvine accessions. The study
revealed that of ISSR primers were efficient in divulging the differences
between Piper species and within P. betle.
ISSR markers were used to identify sex linked markers in
dioecious betelvine. Female and male bulk DNAs were screened with 35
ISSR primers. Two primers viz; ISSR-10 and UBC-852 produced male
specific bands of size 459bp and 1250bp respectively. ISSR-23 amplified
a female specific 636bp fragment. These primers were validated in the
individuals of the bulks and showed a consistent sex specific expression.
A sequence characterized amplified region (SCAR) was developed from
the primer ISSR-23. The SCAR primers developed can further be utilized
in sex identification in betelvine.
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Keywords
genetics, dna, rapd, polymorphism, planting, germplasm, pcr, genetic markers, crops, cloning