PRODUCTION AND OPTIMIZATION OF INDUSTRIALLY IMPORTANT PROTEASE ENZYME FROM EXTREMOPHILES
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Date
2013
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ABSTRACT
The present study was carried out to isolate proteolytic microorganisms from the mushroom compost of Himachal Pradesh, their screening and optimization of protease production under submerged fermentation. Among all the bacterial isolates, bacterial strain C1 and F1 were selected for enzyme production studies depending upon their higher protease producing capability. The selected bacterial isolates C1 and F1 were identified as Bacillus sp and Bacillus licheniformis respectively by physiological, biological and rrs (16S rRNA) PCR technique. Under the submerged fermentation different parameters (i.e. medium, incubation time, pH, temperature, inoculums size, carbon source, nitrogen, metal ions and surfactant) were optimized for the maximum production of extracellular protease. After optimization of different process parameters for Bacillus sp C1, protease titers were enhanced from 1.08 to 7.16IU/ml and percent increase in the enzyme activity had gone upto 842.2% In case of Bacillus licheniformis F1, maximum alkaline protease titers i.e. 7.44 IU/ml were obtained after optimization with 878.94% increase in its enzyme activity. Response surface methodology (RSM) was conducted for optimizing the interactive behaviour of different parameters along with their significance on enzyme yield using Central Composite Design (CCD). Protease activity of 7.21 and 7.50 IU/ml was obtained in Bacillus sp C1 and Bacillus licheniformis F1 respectively. Alkaline protease of Bacillus sp C1 was purified with 7.35 fold, 22.73% yield and final specific activity of 17.35 while Bacillus licheniformis F1 isolate had 5.67 purification fold, 30.50 % yield and specific activity of 14.12 IU/mg.
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enzymes, bacteria, productivity, alkalinity, inorganic acid salts, animal husbandry, amino acids, poultry equipment, fermentation, concentrates, proteolytic microorganisms, mushroom compost of Himachal Pradesh