VARIATION AMONG THE ISOLATES OF Xanthomonas axonopodis pv. malvacearum FROM DIFFERENT LOCATION.
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Date
2021-02-02
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Dr. Punjabrao Deshmukh Krishi Vidyapeeth, Akola, Maharashtra.
Abstract
Bacterial blight of cotton caused by Xanthomonas axonopodis pv. malvacearum one of the most destructive disease of cotton inflicting considerable quantitative and qualitative losses. Mostly the disease occurred on leaves, stems. Considering the economic importance of the crop as well as disease, present study was undertaken to conduct collection of samples, isolation, identification, pathogenicity, study of different characters of pathogen, morphological variability, cultural variability and biochemical variability of selected isolates, measure growth character and colony count on the basis of temperature, pH and salt and in vitro evaluation and sensitivity test of different antiobiotics, fungicides, botanicals, bioagents, against the disease.
The isolation were made from the symptomatic samples collected from different districts of Maharashtra viz. Beed(Xam-1), Parbhani(Xam-2), Akola(Xam-3), Buldhana(Xam-4), Yavatmal(Xam-5), Nanded(Xam-6), Amravati(Xam-7), Aurangabad(Xam-8).
Bacterium X. axonopodis pv. malvacearum was successfully on Nutrient Agar basal culture medium. It produced pale yellow mucoid, shiny, glistering, bacterial colonies.
Pathogenicity X. axonopodis pv. malvacearum was proved by spray inoculation of the bacterial suspension on cotton variety Rashi-679 in pots at ten to twelve leave stage, after predisposition to humid condition for 24hrs. The leaves were inoculated with homogenized culture of X. axonopodis pv. malvacearum. Prior to automization of the culture of leaves were slightly injured with carborendum/celite powder. Seedlings were then retained under humid condition for 24 hrs. by covering the pots with plastics bag. Pots were adequately watered. Simultaneously uninoculated plants were maintained as control.
Cultural and morphological characters and variability such as colony colour and colony shape, colony size, call shape, appearance, colony margin elevation and texture of X. axonopodis pv. malvacearum were studied on NA media.
The studies on biochemical characteristics of X. axonopodis pv. malvacearum showed their positive reactions for potassium hydroxide (KOH) solubility test, Tolerance to 1% NaCl whereas, Negative response for Gram staining, Indole production, and methyl red test.
Measure the growth character and colony count of X. axonopodis pv. malvacearum the basis of different level temperature, different pH level and different salt concentration.
The antibiotic sensitivity against X. axonopodis pv. malvacearum was studied by Paper Disk Method. Streptomycine 300 ppm was significantly superior than all the treatments showing maximum inhibition zone. Whereas antagonistic oraganism such as pseudomonas flouroscens shows the maximum inhibition zone among remaining bioagent. The plant extract neem 10% shows maximum inhibition zone among all botanicals.
Description
The present investigation was carried out at Department of Plant Pathology, Dr. PDKV, Akola during academic year 2018-2020. In vitro evaluation of different chemicals botanicals and bioagents studied by inhibition zone assay method.
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Citation
ANMOD, ANKITA BALAJI. (2020). Variation among the isolates of Xanthomonas axonopodis pv. malvacearum from different location, Department of Plant Pathology Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola. M. Sc. 2020. xiii, 81p. (Unpublished).