GROSS, HISTOLOGICAL AND HISTOCHEMICAL STUDIES ON OVARY, OVIDUCT AND UTERUS IN INDIAN DOMESTIC DOG (Canis lupus familiaris)
Loading...
![Thumbnail Image](assets/images/Item.jpg)
Files
Date
2023-03
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
SRI VENKATESWARA VETERINARY UNIVERSITY TIRUPATI - 517 502. (A.P.) INDIA
Abstract
The female reproductive system in the adult dog consisted of paired ovaries,
oviducts and uterus. Each ovary was caudal to the kidney and hidden inside the ovarian
bursa which opened into the peritoneal cavity by a foramen on the medial surface.
The outermost covering of the ovary was the germinal epithelium of the simple
cuboidal type. The tunica albuginea beneath it contained numerous cortical tubules. The
ovaries showed an outer cortex and an inner medulla. Primordial follicles in the
peripheral part of the cortex beneath the tunica albuginea had and oocyte surrounded by
a single layer of granulosa cells. Primary follicles with the oocyte were lined by a
simple cuboidal epithelium. Secondary follicles with the oocyte were lined by the
stratified epithelium with a glycoprotein layer (zona pellucida) around it. Tertiary
follicles had a large oocyte, zona pellucida, antrum, stratum granulosum and the theca.
The corpus luteum and corpora albicans were also observed. The medulla was centrally
located. The germinal epithelium, tunica albuginea, the zona pellucida and the cells of
corpus luteum were positive for PAS. The tunica albuginea, cortex, membrana
granulosa, theca interna, the capsule and septa of corpus luteum and medulla were
positive for AB.
The oviducts on the surface of the bursa were suspended by a thin mesosalpinx.
Each oviduct contained three parts from cranial to caudal viz, infundibulum, ampulla
and isthmus. The mucosa of the infundibulum formed numerous primary, secondary
and tertiary folds which gradually decreased towards the isthmus. Some of the folds
coalesced and formed cavities in the propria submucosa. The mucosal folds were lined
by a stratified cuboidal epithelium. The tunica muscularis of infundibulum was thin
compared to that in the ampulla and isthmus. Its thickness gradually increased towards
the isthmus. The thin tunica serosa was composed of simple squamous cells and
continued with the mesosalpinx.
The epithelium of mucosal folds and propria-submucosa in infundibulum showed a
positive reaction for AB. The luminal epithelium of the ampulla and isthmus showed a
positive reaction for PAS. Mild PAS positive reaction was observed in the propria
submucosa. The tunica muscularis and tunica serosa showed positive reaction both for PAS
and AB.
The uterus was a thick hollow muscular organ situated in the midline of the
dorsal abdominal cavity. It had a very short body and extremely long narrow horns
which diverged from the body and presented a‟V” shaped structure. The wall of uterine
horn was made up of three layers; endometrium, myometrium and perimetrium from
within outwards. The endometrium was lined by a simple columnar epithelium and had
endometrial glands. The myometrium was composed of inner circular and outer
longitudinal layers of smooth muscle cells with the stratum vasculare in between. The
thickness of endometrium, number of endometrial glands and myometrium gradually
increased from proximal to the distal end. The perimetrium was composed of
mesothelium.
The endometrium of the body of uterus was lined by a simple columnar
epithelium. It was very thick and the glands were numerous and larger. The
myometrium was also thick and the stratum vasculare was the largest among all parts.
The luminal epithelium of the endometrium, its stroma and the uterine glands showed a
positive reaction for PAS. The endometrial glands and myometrium showed a positive
reaction for AB. The endometrium and perimetrium showed a positive reaction for
bromo phenol blue indicating the presence of basic proteins.