CHARACTERIZATION AND IDENTIFICATION OF A VIRUS CAUSING MOSAIC DISEASE IN PEA (Pisum sativum L.)
Loading...
Date
2015
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
ABSTRACT
Surveys conducted during 2014-15 revealed the incidence of mosaic disease of pea (Pisum sativum L.) varying from 5-43 per cent in Solan district of Himachal Pradesh. The characteristic symptoms exhibited by the virus isolates under study were vein clearing, mild mosaic to severe mosaic, rosetting, downward rolling of leaves, tendril shortening, stunted growth of plant bearing distorted pods. Three isolates (I1, I2 and I3) representing different locations namely Nauni, Dharja and Pandah respectively were found to be similar with regard to their reaction on certain indicator hosts (Chenopodium album L., C. amaranticolor Coste, C. murale L., C. quinoa Wild and Vicia faba L.) and serological identity with potyvirus group specific antibodies. The most predominant isolate was then further selected for partial characterization. The isolate was easily transmissible through sap, seed and aphid vectors Acyrthosiphon pisum and Myzus persicae. Rate of seed transmission of virus isolate was 20 percent checked by grow out test and DAC ELISA technique revealed that virus present in seed embryo and not in seed coat. The host range studies revealed that the virus isolate infecteda number of test plants representind the families Chenopodiaceae, Solanacea and Leguminosae. Biophysical properties of the virus isolate included TIP of 55-60°C, DEP of 10-3-10-4 and LIV of 1 day at room temperature (22-26°C). Serological identification of virus isolate through DAC and DAS-ELISA showed positive serological reaction with PSbMV antiserum as well as potygroup specific antisera. The virus isolate had flexuous rod shaped particles and ultrathin section studies revealed the presence of pinwheel, scroll and laminated aggregate inclusions in cytoplasm. The present virus isolate was also successfully detected in RT-PCR assay by using potyvirus group specific primers (CPUP & P9502) and resulted into a desired product of ~700 bp. Hence, based on the results of identification parameters particularly host range, transmission, particle morphology, serological relationship and RT-PCR detection, the present virus isolate has been identified to be similar to PSbMV belonging to potyvirus genus in family potyviridae. Further studies on molecular characterization based on sequencing data of nucleic acid will result in its exact identification and homology with other PSbMV isolates known to infect pea crop. Only two varieties of pea namely Arka Ajit and LC-6 were moderately susceptible, to infection by PSbMV.