In-silico identification of miRNA in Rice (Oryza sativa L.)

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dc.contributor.advisorSudhir Kumar
dc.contributor.authorBhavya
dc.date.accessioned2022-02-22T10:47:55Z
dc.date.available2022-02-22T10:47:55Z
dc.date.issued2020
dc.description.abstractRice (Oryza sativa L.) is the world’s single most important and essential crop and a primary food source for half of the world’s populace. An aggregate of 49% calories consumed by the human population come from rice. Micro RNAs (miRNA) are single-stranded non coding RNA molecules of about 21-23 nucleotides in length which regulate gene expression. The main function of miRNAs is to down-regulate the gene expression. The twelve chromosomes of Oryza sativa was downloaded from the International Rice Genome Sequencing Project (IRGSP), after that they transcribed into RNA and then they were cut into fragments by using split command. The output of split command was subjected as input for palindrome finding in palindrome.pl script. Only four mismatches were allowed. The secondary structures and MFE of output of palindrome.pl script were predicted using RNAfold program. The sequences whose energy were less than -40kcal were sorted and identified as putative miRNA. These putative miRNA were compared against miRNA database in miRBase. Total 61 miRNAs and their 255 putative targets were identified. These target genes have regulatory functions in some certain biological processes.en_US
dc.identifier.urihttps://krishikosh.egranth.ac.in/handle/1/5810182764
dc.keywordsRice, MiRNAs, Nucleotides, Targets, Secondary structureen_US
dc.language.isoEnglishen_US
dc.pages27 + iiien_US
dc.publisherCCSHAU,HiSARen_US
dc.subBioinformaticsen_US
dc.themeIn-silico identification of miRNA in Rice (Oryza sativa L.)Fullen_US
dc.these.typeM.Scen_US
dc.titleIn-silico identification of miRNA in Rice (Oryza sativa L.)en_US
dc.typeThesisen_US
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