STUDY ON SYNERGISTIC EFFECT OF ENDOPHYTIC BACTERIA AND MESORHIZOBIUM IN CHICKPEA (Cicer arietinum)
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Date
2015-07-10
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UNIVERSITY OF AGRICULTURAL SCIENCES GKVK, BENGALURU
Abstract
An investigation was carried out to study the synergistic effect of endophytic
bacteria with Mesorhizobium on chickpea. Large number of endophytic bacteria have
been isolated from different parts of chickpea. Fifteen endophytic bacteria were from
nodules, roots and seeds of chickpea by using nutrient oxide agar media. Different
morphological and biochemical tests were carried out for endophytic bacterial isolates
their compatibility with Mesorhizobium UASB 835 were tested. Among fifteen three
endophytic bacterial isolates were selected based on morphological and biochemical tests
and their compatibility with Mesorhizobium UASB 835 of chickpea. Molecular
characterization was done by using 16S rRNA sequencing for the three compatible
endophytic bacterial isolates. Isolates CPN 4 was identified as Enterobacter sp. CPR 2 as
Enterobacter hormeachie and CPS 1 were identified as uncultured gamma
Proteobacteria. Effect of co-inoculation of endophytic bacteria with Mesorhizobium in
different treatment combination were studied under green house condition using sterilized
soil for their synergistic effect on chickpea. Inoculation of endophytic bacteria and
Mesorhozobium were done by seed treatment and soil drenching method. The significant
difference seen among the treatments. The plant height, nodulation, nodule number,
nodule dry weight, plant biomass, rhizosphere bacterial population and plant nitrogen
content was recorded highest in treatment T13 receiving the inoculation of CPN+ CPR+
CPS+ Mesorhizobium. The results revealed that, co-inoculation of nodule , root and seed
endophytic bacteria with Mesorhizobium on chickpea encourages more robust crop with
better plant health, disease resistance, biomass and ability to cope with environmental
stresses.
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