STUDIES ON QUALITY ASSESMENT OF OOCYTES IN DECCANI BREED OF SHEEP
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Date
2007-04
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SRI VENKATESWARA VETERINARY UNIVERSITY TIRUPATI - 517 502. (A.P.) INDIA
Abstract
ABSTRACT :
The female reproductive tracts of Deccani breed of sheep were procured from slaughter house and studied for the quality assessment of oocytes. The morphology and biometrical characteristics of the ovaries were recorded. The ovaries of non-gravid genitalia differed significantly in weight and breadth with the ovaries of gravid genitalia. All the visible follicles were graded as small (<3mm), medium (3-6mm) and large (>6mm) based on their diameter. Total number of different grades of visible follicles present on the ovary in follicular and luteal phase were 2.35±0.31 and 1.87±0.25 and did not differed significantly. Similarly, the total number of follicles present on right and left gravid ovaries also did not differed significantly. The ovaries thus obtained were subjected to three different techniques of oocyte retrieval, i.e. Aspiration, Dissection and Slicing, respectively. The oocytes retrieved by aspiration, dissection and slicing techniques in follicular and luteal phases were 8.90±0.75, 8.30±0.78, 8.30±0.37 and 5.12±0.72, 5.37±0.63 and 5.37±0.54, respectively. The mean number of A, B, C and D grades of oocytes obtained by these techniques were found to be 1.49±0.13, 1.02±0.11, 1.30±0.12 and 1.46±0.13 respectively and the corresponding values for ovaries collected from the pregnant Deccani ewes were 1.34±0.97, 0.61±.70, 1.06±0.86 and 1.30±0.72, respectively. The length of the ovary was in positive correlation with the oocyte yield in dissection technique (0.370 P<0.05). The mean unit time taken for retrieval of oocytes from ovaries collected from pregnant Deccani ewes for aspiration, dissection and slicing techniques was 269.77±9.69, 500.95±22.05 and 277.29±7.86 seconds and the corresponding values for ovaries collected from non pregnant Deccani ewes were 313.68 ±8.64, 467.59±23.21 and 337.80±14.18 seconds, respectively. The diameter of different grades of oocytes was recorded and the A grade oocytes recorded highest diameter (212.60±1.36 μ) and D grade oocytes recorded the lowest diameter (170.60± 1.48 μ). The diameter of oocytes was found to be gradually decreasing from A grade to D grade oocytes. The thickness of zona pellucida was recorded in the present study and found to be the highest in D grade oocytes (16.20±0.61 μ) and lowest in A grade oocyte (13.80±0.84 μ).
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