SELECTION OF AN EFFICIENT AM FUNGI AND STANDARDIZING THE METHOD OF APPLICATION FOR RICE

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Date
2019
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Acharya N G Ranga Agricultural University, Guntur
Abstract
A pot experiment was conducted to select an efficient AMF for inoculating rice crop from 10 AMF isolates obtained from the rice rhizosphere soil of different agroclimatic zones of Andhra Pradesh. In this study highest plant height (95.80 cm), number of leaves/hill (97.00), tillers and productive tillers per hill (31.67 and 12.00 respectively), shoot and root biomass (15.75 g/hill and 6.30 g/hill respectively), test weight and grain yield (18.26 g and 4520.57 kg ha-1 respectively), N, P, K concentration in straw (0.487%, 0.157% and 1.800% respectively), N, P, K concentration in grain (0.707%, 0.210% and 0.303% respectively), N, P, K uptake in straw (25.30 kg ha-1, 7.96 kg ha-1 and 93.60 kg ha-1 respectively), N, P, K uptake in grain (32.60 kg ha-1, 10.10 kg ha-1 and 13.70 kg ha-1 respectively), available N, P, K (144.54 kg h-1, 32.31 kg ha-1 and 269.61 kg ha-1 respectively), dehydrogenase activity (89.13 µg TPF g-1 soil d-1), acid phosphatase activity (44.23 µg pNP g-1 soil h-1) and alkaline phosphatase activity (67.81 µg pNP g-1 of soil h-1), urease activity (41.50 μg NH4+ g-1 soil h-1), peoxidase activity (45.58 GU g-1 f wt), bacterial population (9.05 × 107 CFU g-1), fungal population (3.10 × 104 CFU g-1), AMF spore load (31.33 spores per 10 g soil), per cent root colonization (47.51%) were highest in the treatments inoculated with Isolate 28 (T8). Based on the above findings Isolate 28 was found efficient among 10 isolates used. The method of application of this isolate was standardized in the second pot experiment. Plant height was highest (116.01cm), number of leaves per hill (149.80), tillers and productive tillers per hill (29.20 and 15.60 respectively), shoot and root biomass (22.33 g/hill and 8.99 g/hill respectively), test weight and grain yield (25.44 g and 6406.90 kg ha-1 respectively), N, P, K uptake in concentration in straw (0.612%, 0.254% and 2.380% respectively), N, P, K concentration in grain (1.224%, 0.260% and 0.488% respectively), N, P, K uptake in straw (45.08 kg ha-1, 18.72 kg ha-1 and 153.24 kg ha-1 respectively), highest N, P, K uptake in grain (78.42 kg ha-1, 16.65 kg ha-1 and xviii 31.26 kg ha-1 respectively),available N, P, K (219.02 kg h-1, 56.32 kg ha-1 and 336.72 kg ha-1 respectively), dehydrogenase activity (121.80 µg TPF g-1 soil day-1), acid phosphatase activity (84.95 µg pNP g-1 soil h-1), alkaline phosphatase activity (94.62 µg pNP g-1 of soil h-1), urease activity (92.94 μg NH4+ g-1 soil h-1), peroxidase activity (66.13 GU g-1 f wt), microbial population of root zone soil, AMF spore load (38.00 spores per 10 g soil) and AMF per cent root colonization (56.60%) was highest in T5 (AMF applied at nursery + Azospirillum, PSB and KRB applied during transplanting). This study clearly showed that isolate 28 (T8) was found efficient as it enhanced the growth and yield, soil microbial population, soil enzymes activity and AMF activity. Hence AMF Isolate 28 can be better utilized along with N, P, K biofertilizers to enhance the growth and yield of rice and AMF should be applied to the nursery.
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