Expression profiling of iron homeostasis gene(s), and antioxidant system in rice (Oryza sativa L.)
| dc.contributor.advisor | Jain, Sunita | |
| dc.contributor.author | Saini, Ritu | |
| dc.date.accessioned | 2017-10-04T09:34:30Z | |
| dc.date.available | 2017-10-04T09:34:30Z | |
| dc.date.issued | 2016 | |
| dc.description.abstract | In the present investigation, biochemical and molecular changes in root and shoot tissues of six rice varieties (Govind, Super, HKR120, Pusa1121, HBC19 and Palman579) differing in grain iron concentration (35- 400 μg/g) were studied in response to varied iron concentrations at vegetative and reproductive stages. Plants were grown in pots in the net house and treated twice with Yoshida solution containing different iron concentrations (0, 0.1 mM, 0.5 mM EDTA-Fe II). Fe content in roots, shoots and grains increased with increase in Fe concentration. Roots of HBC19 and Palman579 and lower and upper shoots of PUSA1121 contained higher iron. Highest iron in dehusked grains was recorded in Palman579 followed by HBC19, PUSA1121, HKR120, Super and Govind. Production of toxic super oxide radical (O2 - ) and hydrogen peroxide (H2O2) and lipid peroxidation (MDA), an indicator of cell membrane damage, enhanced in all the varieties with increase in Fe concentration and increase being higher in the low Fe (Govind and Super) than medium (HKR120 and PUSA1121) and high Fe content varieties (HBC19 and Palman579). Antioxidative metabolites’ contents (ascorbic acid and glutathione) and activities of antioxidative enzymes [super oxide dismutase (SOD), catalase (CAT), peroxidase (POX), ascorbate peroxidase (APX) and glutathione reductase (GR)] invariably increased with increasing iron treatment in both root and shoot. The activities remained significantly low in the low grain Fe content varieties, Govind and Super, as compared to medium and high Fe varieties. A significant positive correlation was observed between the per cent change in H2O2 content and SOD, CAT and POX activities, between percent change in ascorbate and APX and between glutathione content and GR activities. Two SOD, one CAT, three POX and two APX isozymes were common to all six varieties. One more isozyme each of SOD and APX appeared only in medium and high iron varieties. One CAT, two POX and one APX isozymes had differential pattern with respect to varieties and iron treatments in both the tissues and stages while one isoform of SOD, POX and APX was unique to HBC19 shoots at 0.5 mM Fe treatment. Maximum grain yield was recorded at 0.1 mM iron concentration as compared to control and high iron treatment. Relative expression of genes involved in iron homeostasis [Nicotinamine synthase 1 (OsNAS1), Nicotinamine synthase 2 (OsNAS2), Nicotinamine synthase 3 (OsNAS3), Iron-regulated transporter 1 (OsIRT1) and Yellow stripe like 2 (OsYSL2)] was analyzed in Govind, PUSA1121 and Palman 579; expression of these genes except OsYSL2 was higher in root tissues of Govind. In the shoot tissues, expression of OsNAS1 and OsNAS2 was maximum in Govind while that of OsNAS3, OsYSL2 and OsIRT1 was more in PUSA1121 and Palman579. Expression of all the genes except OsYSL2 in root tissues was up-regulated with increase in iron concentration. | en_US |
| dc.identifier.uri | http://krishikosh.egranth.ac.in/handle/1/5810032238 | |
| dc.keywords | Rice, Iron, ROS, Antioxidative defense system, Fe homeostasis genes | en_US |
| dc.language.iso | en | en_US |
| dc.publisher | CCSHAU | en_US |
| dc.sub | Biochemistry | en_US |
| dc.subject | null | en_US |
| dc.theme | Expression profiling of iron homeostasis gene(s), and antioxidant system in rice (Oryza sativa L.) | en_US |
| dc.these.type | Ph.D | en_US |
| dc.title | Expression profiling of iron homeostasis gene(s), and antioxidant system in rice (Oryza sativa L.) | en_US |
| dc.type | Thesis | en_US |