Studies on impact of natural ageing and seed priming on seed quality in Indian mustard [Brassica juncea (L.) czern. & coss
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Date
2018
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CCSHAU
Abstract
The present study entitled “Studies on impact of natural ageing and seed priming on seed quality in Indian mustard. [Brassica juncea (L.) Czern. & Coss.]” was carried out in the Department of Seed Science and Technology at CCS Haryana Agricultural University, Hisar (Haryana) during the year 2015-16 and 2016-17. The experiments was laid out in factorial Complete Randomized Design (CRD) and Randomized Block Design (RBD) for laboratory and field parameters, respectively on three varieties of Indian mustard viz., RH 30, RH 9304 and RH 0406 with three seed lots of each variety. The present investigation was carried out in two different experiments to meet out the objectives of study. In the first experiment, three lots of different Indian mustard were studied for various seed physiological parameters viz., standard germination, seedling length, seedling dry weight, vigour index-I & II, viability test (Tz %), and accelerated ageing. All physiological parameters were decreased significantly with the advancement of natural ageing of seeds. All the biochemical parameters viz., peroxidase (POX), superoxide dismutase (SOD), dehydrogenase enzyme activity decreased significantly with passage of time, whereas, electrical conductivity of seeds leachates increased with the advancement of time. The field parameters viz., seedling emergence index and seedling establishment were also decreased significantly with the advancement of time whereas, mean emergence time increased with the passage of time. In the second experiment, six seed priming treatments viz., T0: Control, T1: hydro-priming followed by dry dressing with carbendazim (2g/kg), T2: Hydration with GA3 (50 ppm), T3: Hydration with KNO3 (0.5%), T4: Hydration with KH2PO4 (0.5%), T5: Biofertilizer (Azotobacter) were analyzed to identify the suitable priming treatment. Among various priming treatments „T2‟- hydration with GA3 @ 50 ppm followed by „T6‟-Biofertilizer performed best to enhance all the seed vigour and viability characteristics and to lower down the electrical conductivity of naturally aged seed lots of Indian mustard seeds. The seed quality improvement through seed priming was noticed more in marginal seed lot i.e. one year old seed lot. Among the different varieties, maximum enhancement was observed in RH-30 followed by RH-9304 whereas, minimum enhancement was observed in variety RH-0406 during the periodof study.
In conclusion, the present study revealed that Indian mustard seed lose its viability and vigour with the advancement of storage time and seed priming with GA3 @ 50 ppm and Biofertilizer (Azotobacter) can be used as an effective tool to enhance vigour and viability of seed.
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