PROTEOMIC STUDIES OF SEED PROTEINS IN RICE (Oryza sativa L.)
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Date
2021
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INDIRA GANDHI KRISHI VISHWAVIDYALAYA RAIPUR (Chhattisgarh)
Abstract
In the present research work, the 40 genotypes of rice (Oryza sativa), were fractionated and characterized based on the protein fraction and the total protein and quantifies with the Coomassie Reagent Protein Assay and biochemical characters (sodium dodecyl sulfate polyacrylamide gel electrophoresis, SDS-PAGE). The research work conduct in the Department of Molecular Biology and Biotechnology, Raipur during 2017-2018.
The research work shows the differences among rice genotypes based on rice fraction (prolamin and glutelin) and total protein. Rice seed protein is localized in two types of protein bodies (PB) PB-Ι and PB- ΙΙ. PB-Ι contains prolamin. Whereas PB-ΙΙ is rich in glutelin and globulins. PB-Ι is less digestive than PB-ΙΙ suggesting that prolamin in PB-Ι is nutritionally less meaningful for humans than glutelin in PB-ΙΙ (Jiang et al. Proteome science, 2014, 12:51). In Glutelin Protein fraction, among of selected genotypes Ir-64 (6.20 mg/g), Gp-145-70 (5.31 mg/g), GP-145-48 (5.17 mg/g) calculated higher percentage. The elaychi verity also having a good percentage level of glutelin which also observe in SDS PAGE. Also in Prolamin protein fraction, Swarna (0.12 mg/g), R-RGM-ATN-47 (0.14 mg/g), R-RHP-IM-84 (0.17 mg/g), IET-24780 (0.21 mg/g) calculated the less percentage than the other selected genotypes. In Percentage of total protein IR-64 (7.66 mg/g), Kalam Gurmatia (7.47 mg/g), GP-145-48 (7.46 mg/g), Kalokuchi (7.31 mg/g) are higher than the other selective genotypes. Also, the 15 verities treated with nitrogen doses with control, 40kgha-1, 60kgha-1, and 100kgha-1, Form the observation 100kgha-1 nitrogen dose more effective than other lower and higher doses respectively. These rice verities mainly having more nutritive value than other selective verities due to the low percentage of prolamin (Hyun et al., 2013). On the prolamin protein fraction SDS page, the 15 kD band is less intense observe in verity WR-2, moti basmati, WR-3, R-RGM-AS-40, and very less intense in verities M-1433, Botaki gurmatia, WR-1, and WR-132 respectively.
In our work, obtained SDS PAGE shows the nunki gurmatia and Botaki gurmatia having low intense glutelin acidic subunits bands. In glutelin protein fraction SDS page, RR-149, M-1433, WR-1, IC-116076, Niwari, WR-73, WR-132, malpa, Surmatia, GP-145-66, R-RGM-AS-41, R-RHP-IN-87, R-RHP-IM-84 and IET-24780. N2 doses rice germplasm show the variation in bands and get less intense in total protein SDS page. In total protein SDS PAGE, as the Nitrogen doses increase the glutelin acidic subunits and prolamin bands goes less intense banding pattern. There are slight changes in the intensity of the bands on the variation on nitrogen observe in SDS PAGE. From the percentage of protein and SDS PAGE banding pattern we conclude, some rice genotypes have more nutritious than other genotypes. As per observation rice genotypes having different nutritional abilities. Overall the IR-64 (6.20 mg/g), GP-145-70 (5.31 mg/g), GP-145-48 (5.17 mg/g) in glutelin protein fraction, Swarna (0.13 mg/g), R-RGM-ATN-47 (0.14 mg/g), R-RHP-IM-84 (0.17 mg/g), IET-24780 (0.21 mg/g) in prolamin protein fraction respectively. These rice genotypes are further used for the enhancement of the nutritional quality of rice.
Description
PROTEOMIC STUDIES OF SEED PROTEINS IN RICE
(Oryza sativa L.)