Hemato-biochemical profile and pharmacokinetic study of enrofloxacin in Badri cattle
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Date
2020-10
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G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand)
Abstract
The present investigation was carried out to study the hematological and serum biochemical profile of the Badri cow and juxtapose it with that of Sahiwal breed. The comparative hematobiochemical study was followed by an attempt to understand unique characteristics of the Badri cattle relating to drug metabolism through a pharmacokinetic study of enrofloxacin. Six healthy,
adult, non-pregnant and non-lactating animals of both Badri and Sahiwal breeds were procured for the hemato-biochemical study. Whereas, the pharmacokinetic study was carried out on the six Badri cattle. The animals were acclimatized to the experimental condition one month prior to the start of study. Ad-libitum access to feed and fresh drinking water was provided to the animals.
For the comparative hemato-biochemical study 2ml blood samples from both breeds was collected from the jugular vein. The analysis of hematological parameters was carried out manually whereas for biochemical parameters the method suggested in the diagnostic kits was used for estimation. The results of the study revealed that the Badri cattle showed numerically higher values
for hematological parameters such as TEC, TLC, Hb and PCV. Furthermore, Badri cattle also differed from the Sahiwal breed with respect to biochemical parameters such as AST, ALP, GGT, serum protein, lipid and mineral profiles. In serum enzymes, the Badri cattle showed significantly (P<0.05) lower values of AST and higher levels of ALP and GGT compared to Sahiwal cattle. In protein profile Badri cattle showed a mean serum globulin level of 3.71 g.dL-1 which was less than that of Sahiwal. The mean total serum cholesterol, HDL-cholesterol and triglyceride values of Badri cattle were significantly (P<0.05) higher than the Sahiwal breed. The Badri cattle also showed serum calcium and phosphorus values of 8.85 and 6.76 mg.dL-1, respectively which was also significantly (P<0.05) higher than the values reported in Sahiwal cattle. For the pharmacokinetic study commercial enrofloxacin (10 % w/v) preparation was administered at a dose rate of 7.5 mg/kg intravenously as a single dose. The plasma samples were collected at regular intervals and subjected to analysis by HPLC after appropriate processing. The results obtained were analysed using a software tool, “PK Solver” to obtain the pharmacokinetic parameters. From the study it was revealed that the Badri cattle showed distribution half-life of 0.11 h, a volume of distribution of 7.63 L.kg-1, an elimination half-life of 4.27 h and a MRT of 5.93 h for enrofloxacin. Whereas, a C max level of 0.12 μg.ml-1 was observed for ciprofloxacin in the current study. According to the results obtained in the pharmacokinetic study an individualized dosage regimen containing a priming dose of 5 mg/kg and a maintenance dose of 4.5 mg/kg over a period of 24 h was suggested for the enrofloxacin in Badri cattle. The results of our current study when compared with the previously available literature on other cattle breeds suggested that the Badri cattle differed with respect to hemato-biochemical and pharmacokinetic properties. So, the calculated dosage regimen for enrofloxacin is recommended for use in this breed.