GENETIC IMPROVEMENT IN GREEN GRAM (VIGNA RADIATA L. WILCZEK) FOR MUNG BEAN YELLOW MOSAIC VIRUS RESISTANCE USING RESISTANT GENE ANALOGUE (RGA) PRIMERS
| dc.contributor.advisor | Nandini, R | |
| dc.contributor.author | MARIYAPPAN, S B | |
| dc.date.accessioned | 2023-01-04T03:29:48Z | |
| dc.date.available | 2023-01-04T03:29:48Z | |
| dc.date.issued | 2022-10-08 | |
| dc.description.abstract | Green gram belongs to the family Fabaceae which is a nutritionally rich crop with easily digestible protein. Yield losses in mungbean due to Mungbean Yellow Mosaic Virus (MYMV) is recorded upto 100% which are the major constraints in production. Hence, present investigation was undertaken to study the genetic variability for yield and yield related traits and to identify sources of resistance to MYMV using RGA primers inAdvanced Breeding Lines and germplasm during 2019-2021. The analysis of variance revealed statistically significant differences among the genotypes for eight quantitative characters and high heritability coupled with high genetic advance was recorded for seed yield per plant, pods per plant and 100 seed weight indicating the involvement of additive gene action. A strong positive correlation and significant direct effect was observed between seed yield, pod length, pods per plant, seeds per pod and plant height. On screening mungbean genotypes under natural field conditions for MYMV resistance, 25 of them were found to be resistant, 222 moderately resistant and 53 were moderately susceptible, while the 2 checks were susceptible based on DI, PDI and AUDPC. However, when the genotypes were screened using 4 RGA primers, VMR1 amplified in 24 genotypes at 445bp and CYR1 primer amplified in 13 genotypes at 1236bp. 24 genotypes were further screened under hot spot condition for MYMV resistance and found to be stable resistant source. Thus,13 genotypes which amplified in both primers taken as testers and 10 susceptible genotypes taken as lines. Combining ability analysis of crosses revealed significant variability for all the characters in parents and among crosses. Further, GGABL- 78 X GG-ABL-200 and GG-ABL-101 X GG-ABL-189 were identified as promising crosses based on per se performance, sca effects and standard heterosis and it can be utilized for isolation of transgressive segregants in further breeding programme | en_US |
| dc.identifier.other | Th-13280 | |
| dc.identifier.uri | https://krishikosh.egranth.ac.in/handle/1/5810191110 | |
| dc.keywords | VIGNA RADIATA L WILCZEK | en_US |
| dc.language.iso | English | en_US |
| dc.pages | 241 | en_US |
| dc.publisher | University of Agricultural Sciences, Bangalore | en_US |
| dc.sub | Genetics and Plant Breeding | en_US |
| dc.theme | MUNG BEAN YELLOW MOSAIC VIRUS RESISTANCE USING RESISTANT GENE ANALOGUE (RGA) PRIMERS | en_US |
| dc.these.type | Ph.D | en_US |
| dc.title | GENETIC IMPROVEMENT IN GREEN GRAM (VIGNA RADIATA L. WILCZEK) FOR MUNG BEAN YELLOW MOSAIC VIRUS RESISTANCE USING RESISTANT GENE ANALOGUE (RGA) PRIMERS | en_US |
| dc.type | Thesis | en_US |