BACTERIOLOGICAL STUDIES ON PREPUTIAL WASHINGS AND AT VARIOUS SUBSEQUENT STAGES OF SEMEN PROCESSING IN COW BULLS
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Date
2019-05-17
Authors
Rana, Pankaj
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CSKHPKV, Palampur
Abstract
The objective of the present study was to quantify the bacterial load at various stages of its collection and processing viz. preputial washings, neat semen after collection and further extended and frozen semen post-thaw. Samples were collected twice, each from 15 bulls, at weekly interval at all the stages of collection and processing. Four egg yolk and 4 extender samples were also collected. Samples were cultured over Plate Count Agar (PCA) for estimation of bacterial load. Bacterial isolation was done over blood agar, nutrient agar, EMB agar, MLA and BGA agar. Various gross, microbiological and biological tests were used to identify the isolates. Muller Hinton Agar (MHA) was used for antibiogram of isolates.
Of all 124 samples processed, per cent of samples found positive for bacterial presence were 100, 100, 73.33, 43.33, 75 and 0 in preputial washings, neat semen, extended semen, frozen semen straws, egg yolk and extender, respectively. The mean bacterial load per ml of different ejaculates (n=30) consisting of preputial washings, neat semen, extended semen and frozen semen post-thaw were 166608.00±28351.80 (15000-868750), 4107.31±433.04 (750-10100), 116±33.39 (0-600) and 29.00±15.51 (0-400) CFU/ml, respectively.
The predominant organism isolated were Staphylococcus spp. and Bacillus spp. in preputial washings and Staphylococcus spp. and Streptococcus spp. in neat, extended and frozen semen.
Bacterial isolates were found to be sensitive to streptomycin, ciprofloxacin and marbofloxacin (100% each), gentamicin (93.33%), enrofloxacin (93.33%), ofloxacin (80.0%), ampicillin (40.0%) and lincomycin (39.99).
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