STUDIES ON IDENTIFICATION, CHARACTERIZATION AND EFFECTIVENESS OF Trichoderma spp. AGAINST CHICKPEA SOIL BORNE PATHOGENIC FUNGI
| dc.contributor.advisor | S. KHAYUM AHAMMED | |
| dc.contributor.author | MADDI SANDHYA | |
| dc.date.accessioned | 2023-12-26T14:09:27Z | |
| dc.date.available | 2023-12-26T14:09:27Z | |
| dc.date.issued | 2023-12-26 | |
| dc.description.abstract | In the present investigation on “Studies on identification, characterization and effectiveness of Trichoderma spp. against chickpea soil borne pathogenic fungi”, three soil borne pathogenic fungi i.e., Sclerotium rolfsii, Rhizoctonia bataticola and Fusarium oxysporum f. sp. ciceri symptoms were collected from the infected fields of chickpea and were isolated. Thirteen Trichoderma isolates were collected from the Department of Plant Pathology, Agricultural College, Bapatla. Of all the 13 Trichoderma isolates, four isolates were considered as potential isolates and further assessment was made to study their variability in morphological, cultural, biochemical and molecular characters. They were also tested for their sensitivity towards various fungicides and insecticides. Variability studies of thirteen Trichoderma isolates collected were performed using cultural characters such as colony texture, elevation, growth habit, colony margin and mycelial nature. All Trichoderma isolates were grown on PDA to study their morphological and cultural variability and grouped into four categories i.e., very fast, fast, medium and slow based on radial growth. Isolate T 19002 showed fast growth (4.1-6.0 cm) and remaining 12 isolates with medium growth of 2.1 to 4.0 cm range at 1 day after incubation (DAI). At 3 DAI, T 19001 and citrus isolates showed fast growth and remaining 11 isolates showed very fast growth (>6.1 cm) and considered to be having better proliferation and all isolates showed very fast growth by 5 DAI. Observations at 3 DAI indicated that most of the isolates recorded growth rate between 0.5 to 1.0 mm/hr. xvii Based on bioefficacy studies in vitro using dual culture technique against S. rolfsii, R. bataticola and Foc. The present investigation revealed that incubation up to five days was insufficient to categorize an isolate as potential. Hence, based on other qualitative parameters such as zone of inhibition, overgrowth potential, lysis, sporulation in Trichoderma were also considered for screening and selection of potential isolates. Isolates (T 19006, T 19015, T 19002, T 19026) with fast growth, lysing and overgrowing with good sporulation over test pathogen at zone of inhibition (zi), pigmentation were considered as potential isolates against all three test pathogens i.e., S. rolfsii, R. bataticola, Foc and used for further studies. The four potential Trichoderma isolates were assessed for their variabililty in morphological, cultural, biochemical, molecular characterization and their sensitivity in radial growth and spore germination. Based on morphology of conidiophore branching, phialides shapes, the 4 Trichoderma isolates were identified as T. harzianum (T 19006, T 19015), T. asperellum (T 19002), T. aureoviride (T 19026). The four potential Trichoderma isolates were assessed for their variabililty in morphological, cultural, biochemical, molecular characterization and their sensitivity in radial growth and spore germination. Based on morphology of conidiophore branching, phialides shapes, the 4 Trichoderma isolates were identified as T. harzianum (T 19006, T 19015), T. asperellum (T 19002), T. aureoviride (T 19026). Assessment of molecular variability using SSR primers for 4 potential Trichoderma isolates was performed which resulted in 60 bands with polymorphism having band size from 100 to 2000 bp. Where, some of the primer sequence was used to identify Trichoderma genus upto species level and confirmed them as T. asperellum, T. aureoviride and T. harzianum. Dendrogram obtained had 2 clusters (I and II). Cluster I consists of 3 isolates and cluster II contain I isolate (T19002), had a similarity of 53 per cent with each other cluster. Biochemical studies were performed to test their chitinase and β-1,3-glucanase activity, where banana isolate have high enzyme production i.e., 181.54 mg ml-1 and 122.06 μmol min-1 ml-1 respectively. Among six insecticides tested for their sensitivity by poison food technique, spinosad completely inhibited the radial growth of all four Trichoderma isolates at all the three concentrations (R/2, R and 2R respectively). Indoxacarb showed mycelial growth only at R/2 concentration and complete inhibition was observed both at R and 2R concentrations in all Trichoderma isolates. Among six fungicides tested for their sensitivity by using slide germination technique, metalaxyl + mancozeb, azoxystrobin + tebuconazole, carbendazim + mancozeb, azoxystrobin + difenconzole, carboxin + thiram complete inhibition of spore germination in four potential Trichoderma isolates at all the three concentrations (R/2, R and 2R) was observed and tebuconazole+trifloxystrobin showed spore germination with significant difference when compared to control. Among six insecticides tested for their sensitivity, spinosad completely inhibited the spore germination of all Trichoderma isolates followed by profenophos and rynaxypyr at all the three concentrations (R/2, R and 2R respectively). | |
| dc.identifier.other | D6452 | |
| dc.identifier.uri | https://krishikosh.egranth.ac.in/handle/1/5810204468 | |
| dc.keywords | IDENTIFICATION | |
| dc.keywords | CHARACTERIZATION | |
| dc.keywords | EFFECTIVENESS | |
| dc.keywords | Trichoderma | |
| dc.keywords | CHICKPEA SOIL | |
| dc.language.iso | English | |
| dc.pages | 210 | |
| dc.publisher | Acharya N G Ranga Agricultural University | |
| dc.relation.ispartofseries | D6452; D6452 | |
| dc.sub | Plant Pathology | |
| dc.theme | STUDIES ON IDENTIFICATION, CHARACTERIZATION AND EFFECTIVENESS OF Trichoderma spp. AGAINST CHICKPEA SOIL BORNE PATHOGENIC FUNGI | |
| dc.these.type | M.Sc | |
| dc.title | STUDIES ON IDENTIFICATION, CHARACTERIZATION AND EFFECTIVENESS OF Trichoderma spp. AGAINST CHICKPEA SOIL BORNE PATHOGENIC FUNGI | |
| dc.type | Thesis |