STUDIES ON IN VITRO REGENERATION IN PUMMELO {Citrus grandis fL.] Osb.)
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Date
2008-03-13
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University of Agricultural Sciences GKVK, Banglore
Abstract
Studies on in vitro regeneration of an elite genotype of pummelo (Citrus grandis
[L.] Osbeck.) revealed that 0.75 mg/1 was the best concentration of benzyl adenine (BA)
for induction of multiple shoots from in vitro nodal explants. Full strength MS medium
showed its overall superiority over half strength MS, MT, and WPM media for shoot
multiplication from the same explants. While NAA did not yield satisfactory root
induction in microshoots, IBA at 4 mg/1 was found to be the best treatment which
induced cent per cent rooting in microshoots accompanied with normal shoot growth. For
plantlet regeneration through indirect organogenesis, 2,4-D induced better callus
formation on cotyledon explants than NAA, quantitatively and qualitatively.
Combination of 1 or 2 mg/1 2,4-D + 1 mg/1 BA was observed as the best treatment for
callusing. While NAA-induced calli failed to regenerate shoots, 2,4-D-induced calli
regenerated shoots on the media containing 5 mg/1 BA with frequency of 90 per cent and
average of 1.9 shoots per explant. Adenine sulphate appeared less effective than BA in
induction of shoots from the calli. For indirect somatic embryogenesis, ovary tlCLs
explants showed the highest callus formation of 80 per cent on the medium supplemented
with 3 mg/1 BA + 500 mg/1 ME. These calli could not produce embryo on embryo
induction media. Immature ovule explants produced callus with frequency of 81 per cent
on media containing 2,4-D at 0.2 mg/1. Media containing 2,4-D was superior than the
ones containing NAA or BA in callus induction from immature ovules. Transferring of
the 2,4-D- and NAA-induced calli on the media supplemented with different
carbohydrate sources, i.e. sucrose, maltose, galactose and lactose each at 30 and 50 g/1
could not induce somatic embryogenesis in the calli. Supplementation of sucrose-based
media with 2,4-D, 2,4-D plus kinetin, ABA, TDZ, and L-glutamine each at different
concentrations also did not yield somatic embryogenesis.
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