Dietary Manipulation Of Fat On Mitochondrial Decay And Its Implications On Ageing And Apoptosis

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dc.contributor.advisorLeela, V.
dc.contributor.advisorThangavel, A.
dc.contributor.advisorMathuram, L.N.
dc.contributor.advisorVairamuthu, S.
dc.contributor.authorDavid, Corbon Godfrey, I.
dc.contributor.authorTANUVAS
dc.date.accessioned2016-05-30T15:32:14Z
dc.date.available2016-05-30T15:32:14Z
dc.date.issued2009
dc.description.abstractAn investigation was carried out to study the effect of dietary manipulation of fat and calorie restriction (CR) on the production of ROS in relation to activity of mitochondrial electron transport complexes and its implications on apoptosis in ageing heart and brain. Eighteen months old male Wistar Albino rats were fed on coconut and corn oil based diets ad libitium or at 40 per cent CR up to 24 (short term) or 30 months (long term) of age. At the end of feeding trial rats were sacrificed. Mitochondria from heart and brain were isolated for the estimation of membrane fatty acid composition, H2O2 and OXPHOS enzyme activity. Mitochondrial inner membrane surface area was measured stereologically from electron micrographs. Antioxidant enzyme status, markers of oxidative stress measured as TBARS and protein carbonyls and DNA fragmentation were measured in both the tissues. Myocardial collagen and serum lipid profile were also estimated to correlate the effect of dietary fat and CR on retarding ageing. Both short and long term feeding of linoleic acid low coconut oil and linoleic acid rich corn oil based diets with CR increased oleic (18:1) linoleic acid (18:2) levels respectively in the heart and brain mitochondrial membranes. Reinforcement of 18:1 levels in membranes increased inner membrane surface area, membrane potential and reduced H2O2 production in rats fed on coconut oil based diet with CR compared to corn oil diet. The oxidative enzyme activities did not show appreciable age related variation in rats fed on coconut oil and corn oil based diets. Although, short term feeding of both coconut oil and corn oil based diets with CR effectively minimized age related changes in heart and brain, on the long run coconut oil based diet with CR was found effective to reduce oxidative damage to lipids and proteins but not DNA through enhanced antioxidant status. It is concluded that coconut oil based diets with CR reduces age related changes in heart and brain through reinforcement of membrane fatty acids, improved membrane integrity and decreased oxidative stress.en_US
dc.identifier.urihttp://krishikosh.egranth.ac.in/handle/1/66458
dc.language.isoenen_US
dc.publisherTamil Nadu Veterinary and Animal Sciences Universityen_US
dc.subVeterinary Physiology
dc.subjectCalorie restrictionen_US
dc.subjectDietary faten_US
dc.subjectCoconut oilen_US
dc.subjectCorn oilen_US
dc.subjectMitochondriaen_US
dc.subjectOxidative stressen_US
dc.subjectAntioxidant statusen_US
dc.subjectApoptosisen_US
dc.subjectAgeingen_US
dc.subjectHearten_US
dc.subjectBrainen_US
dc.these.typePh.D
dc.titleDietary Manipulation Of Fat On Mitochondrial Decay And Its Implications On Ageing And Apoptosisen_US
dc.typeThesisen_US
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