EVALUATION OF CALCIUM AND SILVER NANOCOMPOSITE FILMS FOR CUTANEOUS WOUND HEALING IN RABBITS
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Date
2011
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Sri Venkateswara Veterinary University, TIRUPATI – 517 502,A.P
Abstract
ABSTRACT :
The present study was conducted on eighteen rabbits divided into three groups of six animals comprising group-I with control group of animals, group-II with calcium-silver (35:55) nanocomposite films applied animals, group-III with calcium-silver (45:45) nanocomposite films applied animals. Cutaneous wounds were created at the loin region of all the animals and the wounds were left unsutured in control group, same size nanocomposite films were cut and applied in group-II and group-III animals. These were evaluated by clinical, physiological, gross, haematological, biochemical and histological studies. Group-III animals showed early healing compared to group-I and group-II. These nanocomposite films did not alter the physiological parameters.
The total erythrocyte count values showed a significant decrease followed by significant increase in group-I, significant and nonsignificant increase throughout the period of observations was seen in group-II and group-III respectively. The changes were significant among the groups on day-0, day-1 and day-2. Haemoglobin values differed significantly throughout the observation intervals in group-I and group-II. Significant difference was observed among the groups on day-0, day-1 and day-2. PCV values showed significant changes between different observation intervals in group-I and group-II but the changes were nonsignificant in group-III. The values differed significantly among the groups on day-1 and day-7. The total leucocyte count values showed a significant increase followed by significant decrease reaching almost initial value by day 14. There was a significant difference between the groups and also among the groups. In differential leucocyte count, neutrophil count and lymphocyte count showed significant difference between the groups and also among the groups. The esinophil, monocyte and basophil counts in all the groups did not show any significant changes among the groups and so also between the groups.
Hydroxyproline and all trace elements showed significant changes among the groups and also between the groups with lower value on day 7 and higher value on day 14. Hexosamine values showed significant changes between the groups and also among the groups but with higher value on day 7 which were decreased on day 14. None of the animals showed a positive reaction for C- reactive protein at different periods of observations in all the groups. Early fibroplasia, vascularization and epithelialisation were noticed in group III compared to other two groups.
Based on the observations in the present study it was concluded that calcium-silver nanocomposite films could be used safely for cutaneous wound healing without any adverse effects.
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Keywords
RABBITS ; CALCIUM-SILVER; NANOCOMPOSITE FILMS; CUTANEOUS WOUND