Studies on Pathology and Immune Response against Moniliformin in Rats
| dc.contributor.advisor | Asrani, R.K. | |
| dc.contributor.author | Roy, Suman | |
| dc.date.accessioned | 2019-02-02T10:00:03Z | |
| dc.date.available | 2019-02-02T10:00:03Z | |
| dc.date.issued | 2013-06-28 | |
| dc.description.abstract | A total of thirty six adult Sprague-Dawley rats were taken for the present study, which have divided into three groups viz. CX, LX and HX, containing twelve rats in each group. Rats in CX group were fed normal rat diet alone, whereas rats in LX and HX group were fed 100 ppm and 200 ppm moniliformin (M) from Fusarium fujikuroi M-1214 culture material (MCM) containing 10,000 mg M per kg culture material, respectively. Diets were fed from day 1 to day 42 and the parameters of study included mortality, clinical signs, growth response, behavioural changes, daily feed and water intake, serum biochemical changes, cellular and humoral immune responses, electrocardiographic alterations, and gross, microscopic and ultra structural pathological changes. Four rats from each group were sacrificed at day 21 and rest at day 42. Rats in both toxin fed groups showed no mortality and reduction of body weight, feed and water intake. Nervous excitement and localized alopecia were seen in the rats of group HX only. Total serum protein, creatine kinase were increased in both toxin fed groups compared with the CX group. Cell mediated immune response was found to be higher in both the toxin fed groups as compared with CX group associated with increment of the thickness of ear pinna and mononuclear cellular infiltrations. Humoral immune response was highest in LX group associated with highest log10 antibody titer against sheep RBCs compared with other two groups. Electrocardiographic findings revealed mild degree of tachycardia and hypertrophy in both the toxin fed groups associated with decreased RR-interval and increased Ramplitude. Grossly, dilatation and hypertrophic ventricular wall were evident in both the toxin fed groups in a dose-dependent manner. Microscopically, myocardial karyomegaly, nuclear pleomorphism, hyperchromasia, myofibril disarray exhibiting wavy pattern, hypertrophy of myofibers, degenerative changes viz. vacuolation, loss of cross-striation and increased sarcoplasmic granularity were revealed in both the toxin fed groups in a dose-dependent and time-dependent manner. Liver, kidney and showed mild changes as cellular swelling, mild bile duct hyperplasia, goblet cell hyperplasia and cellular infiltrations. Transmission electron microscopy study in heart revealed maximum effect of M toxicity on the mitochondria. In addition, increased number and pleomorphism of mitochondria and the mitochondria were invariably swollen although the outer membrane was intact. Partial loss of banding pattern due to accumulation of mitochondria and increased peri-nuclear aggregation of mitochondria were the consistent findings in both toxin fed groups. It is thus concluded that M is cardiotoxic in rats though it potentiates immune response of the body which was significantly better at 100 ppm dose level at 42 days of M feeding. | en_US |
| dc.identifier.uri | http://krishikosh.egranth.ac.in/handle/1/5810093401 | |
| dc.keywords | Rats | en_US |
| dc.language.iso | en | en_US |
| dc.pages | 73 | en_US |
| dc.publisher | CSKHPKV, Palampur | en_US |
| dc.sub | Veterinary Pathology | en_US |
| dc.subject | null | en_US |
| dc.theme | Pathology and immune response against moniliformin in rats | en_US |
| dc.these.type | M.V.Sc. | en_US |
| dc.title | Studies on Pathology and Immune Response against Moniliformin in Rats | en_US |
| dc.type | Thesis | en_US |