STUDIES ON THE TRANSFERABILITY AND EFFICIENCY OF DIFFERENT DNA MARKER SYSTEMS FROM OTHER VIGNA SPECIES IN MUNG BEAN (Vigna radiata (L.) Wilczek)

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dc.contributor.advisorRAMANA, J.V.
dc.contributor.authorMANORAMA, KESINENI
dc.date.accessioned2020-01-25T08:51:38Z
dc.date.available2020-01-25T08:51:38Z
dc.date.issued2019
dc.descriptionD5786en_US
dc.description.abstractThe present investigation was carried out at MBB lab, Advanced Post Graduate Centre, Lam, Guntur, Andhra Pradesh. Mung bean (Vigna radiata (L.) Wilczek) is a self-pollinated crop. It is a diploid grain legume (2n=22) with a genome size of 579 Mbp. It is a good substitute for meat in most Asian diet and a significant component of various cropping system. To characterize 38 genotypes of mung bean using SSR, RAPD and ISSR markers to study the transferability, efficiency and polymorphism. The study was to examine the transferability of SSR, RAPD and ISSR markers in mung bean from related Vigna spp and study their validity in efficiency analysis. Sixty four markers were used which includes forty six SSR, nine RAPD and nine ISSR. Forty seven markers (thirty two markers of SSR, nine primers of RAPD and six primer of ISSR) out of the sixty four markers were amplified from other Vigna species and transferable to mung bean. Thirty two primers of SSRs which were transferred to mung bean were from other Vigna species. Adzukibean, Blackgram, Chickpea, Cowpea and Rice bean showed 89, 100, 40, 70 and 67 per cent transferability, respectively. The range of transferability across Vigna species was 67-100%. The range of transferability across mung bean genotypes was 7- 100%. Nine primers of RAPD which were transferred to mung bean were from other Vigna species have shown 100% transferability to mung bean. Nine RAPD markers were transferable which yielded fifty nine alleles with an average of 6.6 alleles per locus. The transferability range across mung bean genotypes was 73.7 – 100%. Six primers of ISSR which were transferred to mung bean were from other Vigna species have shown the transferability range 50- 100%. The range of transferability across mung bean genotypes was 92 - 100%. xiv Expected heterozygosity of SSR, RAPD and ISSR marker ranges from 0.05- 0.5, 0.491 and 0.145-0.499 respectively. Average heterozygosity of SSR, RAPD and ISSR is 0.256, 0.113 and 0.132.respectively. Average EMR ratio for SSR, RAPD and ISSR markers 2.03, 4.676 and 2.57 respectively. Average marker index value for SSR, RAPD and ISSR was 0.57, 1.73 and 0.834 respectively. CEDG103, CEDG022 and CaGM01514 markers were mostly monomorphic among all the mung bean genotypes under study. Most markers were polymorphic which was less than 5 i.e. least informative. OPN 9 primer of blackgram showed unique allele at 900 bp in Pusa Baisakhi. Jaccard’s similarity coefficient ranged for SSR markers from 0.61 to 0.88. Highest JSC value in cluster I and lowest JSC value in cluster IV. Jaccard’s similarity coefficient for RAPD ranged from 0.6 - 1. Highest JSC value in cluster I and lowest JSC value in cluster IV. Jaccard’s similarity coefficient for ISSR ranged from 0.56 - 1. Highest JSC value in cluster I and lowest JSC value in cluster III. In conclusion, RAPD and ISSR were multi locus marker systems expected to produce higher EMR and MI values were efficient than single locus (SSR) marker systems. RAPD and ISSR were good in the detection of polymorphism and also efficient marker systems. Similarity coefficients were same for multi locus marker systems (ISSR and RAPD) which is low than single locus marker systems (SSR). Finally they indicated narrow genetic base of tested green gram landraces. Breeding between the genotypes (intraspecific) in mung bean shows less variation. So, better to go for interspecific breeding to develop variation.en_US
dc.identifier.urihttp://krishikosh.egranth.ac.in/handle/1/5810142083
dc.keywordsTRANSFERABILITY, DIFFERENT DNA MARKER SYSTEMS, MUNG BEAN (Vigna radiata (L.) Wilczek)en_US
dc.language.isoen_USen_US
dc.pages102en_US
dc.publisherAcharya N G Ranga Agricultural University, Gunturen_US
dc.research.problemSTUDIES ON THE TRANSFERABILITY AND EFFICIENCY OF DIFFERENT DNA MARKER SYSTEMS FROM OTHER VIGNA SPECIES IN MUNG BEAN (Vigna radiata (L.) Wilczek)en_US
dc.subBiotechnology and Molecular Biology
dc.subjectnullen_US
dc.themeSTUDIES ON THE TRANSFERABILITY AND EFFICIENCY OF DIFFERENT DNA MARKER SYSTEMS FROM OTHER VIGNA SPECIES IN MUNG BEAN (Vigna radiata (L.) Wilczek)en_US
dc.these.typeM.Scen_US
dc.titleSTUDIES ON THE TRANSFERABILITY AND EFFICIENCY OF DIFFERENT DNA MARKER SYSTEMS FROM OTHER VIGNA SPECIES IN MUNG BEAN (Vigna radiata (L.) Wilczek)en_US
dc.typeThesisen_US
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