EVALUATION OF CRACKING AND NON - CRACKING GENOTYPES OF JACK AND IDENTIFICATION OF SCAR MARKER RELATED TO FRUIT CRACKING
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Date
2008-07-29
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University of Agricultural Sciences GKVK, Bangalore
Abstract
The investigations on “Evaluation of cracking and non-cracking genotypes
of Jack and identification of SCAR marker related to fruit cracking” were
carried out at the Plant Molecular Biology Laboratory, Division of Horticulture,
UAS, GKVK, Bangalore-65, during the period 2007-2008.
A survey was conducted in the orchards of Department of Horticulture, Farm
Superintendent Office of UAS, Bangalore and State Department Farm, Tamaka at
Kolar District. Study employed morphological and molecular markers for
differentiating cracking and non-cracking type. Of all the cultivars used in the
survey, the cultivar T-10 was the tallest. TCJ-03 produce highest number of fruits
(58). The other parameters like stem thickness (120cm) and number of branches (20)
were also highest in TCJ-03. The quality parameters in term of TSS was higher,
acidity was low (0.04%) with moderate pectin (2.89%) in the cultivar TCJ-04 as
compare to other.
The RAPD analysis identified OPC-07 as the polymorphic primer
showing band size of 810 bp as the polymorphic marker. The sequence data of
fruit cracking character was analysed for its identity using BLASTX search for its
homology with the sequence of a gene already recorded in the database of the
NCBI, Bethesda, USA. Nucleotide sequence data reported here have been deposited
in Gene Bank (NCBI) under the accession numbers –
dbEST-ld User-ld GeneBank-Accn
56934622 Jackfruit 1 FG 228207
The sequence of the marker associated with fruit cracking as follows
GTCCCGACGAGCAAAAGCTGGATTGGCAAAAAACTTGAGTTGCATATTACCGCGTTGGG
TAAGGCATTATTAGCCTGGAAGACACGAGATGAACTGGATTATTTTTTAGAAGCACTCA
CGTTAACTCCTCATACGCGCAATACATTTACCGATAAAAAATTGTTTCTGGAAGAATTAC
AAAAAACACGCCTTCGGGGATGGGCCATAGACAACGAAGAATCAACCTATGGGGCGGT
ATGTTTAAGTATGCCGGTATTCAATATGTATAGCAGAGTTAACTATGCAATCTCGCTTTC
TGGCGATCCGGTAGTTTATTCAGGAAATAAGATAGACAGCTATCTGGAATTGCTCCGGA
AATGCGCTGAGCAAATATCATATGGGCTGGGATACAGAAACGAAAATGAGCACTTACG
AAAAGGAAACTGAGGTAATGAAAAAATTCAGCGGCATTATTCCACCGGTATCCAGCACG
TTTCATCGTGACGGAACCCTTGATAAAAAGCAATGCGCGAAGTTGCCGACTTCCTGATTA
ATAAAGGGGTCGACGGGCTGTTTTATCTGGGTACCGGTGGTGAATTTAGCCAAATGAAT
ACAGCCCAGCGCATGGCACTCGCCGAAGAAGCTGTAACCATTGTCGACGGGCGAGTGCC
CGGTATTGATTTGGCGTCGGTTCCCCTTCCACTGACGAAGCGGTCAAACTGGCGCAGCAT
GCGCAAGCCTACGGCGCTGATGGTATCGTCGGGAC
In the present study, these findings illustrated the usefulness of molecular
markers as a tool for the crop improvement programs in jackfruit by identifying the
marker associated with fruit cracking which can be used for identification of
cracking types in the seedling stage itself.
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