STUDIES ON THE HEPATOPROTECTIVE EFFECT OF SARDINELLA LONGICEPS FISH EXTRACT AND FLAVONOID QUERCETIN IN RATS
| dc.contributor.advisor | SRIVIDYA, G(MAJOR) | |
| dc.contributor.advisor | RAVI KUMAR, P | |
| dc.contributor.advisor | SUDHAKAR, K | |
| dc.contributor.author | SIMRAN KOUR, S | |
| dc.date.accessioned | 2023-06-30T12:30:57Z | |
| dc.date.available | 2023-06-30T12:30:57Z | |
| dc.date.issued | 2023-02 | |
| dc.description | THESES | en_US |
| dc.description.abstract | In recent years there has been an increased research interest to develop therapeutic agents from natural sources. Fish serve as natural sources of medicines for cardiac disorders due to their richness in polyunsaturated fatty acids. Exploring the therapeutic potential of plant sources such as volatile oils, herbs phytochemicals was studied but limited research reports are available on the therapeutic potential of the fish extract on liver damage. Hence, the present study was designed to determine the effect of Sardinella longiceps fish extract and flavonoid quercetin on liver damage produced by carbon tetrachloride in male albino Wistar rats. Fish possess high protein content and also omega fatty acids known to support good health. The Indian subcontinent harbors a rich fish biodiversity which markedly varies in their nutrient composition. The Indian Oil Sardine (Sardinella longiceps) is a species of ray-finned fish of the genus Sardinella and is the most common species that forms the mainstay of the pelagic fishes of India. It has the highest lipid value (38%) and PUFAs which significantly regulate cell proliferation, and fatty acid metabolism, and in reducing inflammation and oxidative stress. Quercetin (3, 5, 7, 3, 4-pentahydroxy flavon), is a flavonoid considered one of the most prominent dietary antioxidants and occurs in a glycosylated form in French beans, broccoli, apples, and especially in onions. Male Wistar rats weighing 150-200 g, were randomly distributed into five groups, the first served as control and received 1% DMSO orally for 3 weeks and olive oil i.p. twice a week in the 2nd and 3rd week while groups II, III, IV, and V rats received carbon tetrachloride @ 1ml/kg body weight in olive oil (1:1) i.p. twice a week in the 2nd and 3rd week. The third and fourth groups were supplemented with Sardinella longiceps extract @ 300mg/Kg body weight and quercetin @ 30mg/Kg body weight orally daily for 3 weeks, respectively. Rats of group five were exposed to coadministration of Sardinella longiceps extract @ 300mg/Kg body weight and quercetin @ 30mg/Kg body weight orally daily for 3 weeks. Twenty-four hours after the last day of treatment blood samples were collected for estimation of indicators of hepatic damage and liver samples were collected for gross and histopathological examination. Administration of CCl4 for 21 days produced liver injury that was evident from elevated levels of serum enzymes such as ALT, AST, ALP, bilirubin, BUN, creatinine and histopathological alterations such as vacuolar degeneration of hepatocytes, and infiltration of inflammatory cells in sinusoidal space. The CCl4 treatment caused significant (p<0.01) changes in the cellular redox status as reflected by about a 79% decrease in the level of GSH, a marked decrease GPx, SOD, and catalase levels, and an increase in MDA in the liver. Sardinella longiceps extract (300mg/kg orally, daily ) markedly prevented the rise in serum enzyme levels elicited by CCl4. The histopathological alterations were also improved. Remarkably, there was a reversal of the changes to some extent in the levels of GSH and MDA, in animals receiving quercetin. The group receiving the coadministration of Sardinella longiceps extract and quercetin showed better protection against CCl4 and this was evident with improved body weight gain, decreased serum enzyme levels, and less severe histoarchitectural changes in the liver. In conclusion, the present study provided new insights into the use of fish as PUFA supplementation and the combination treatment was the most promising hepato protective formula among the different treatments. | en_US |
| dc.identifier.uri | https://krishikosh.egranth.ac.in/handle/1/5810197895 | |
| dc.keywords | HEPATOPROTECTIVE EFFECT; SARDINELLA LONGICEPS; FISH EXTRACT; FLAVONOID QUERCETIN; RATS | en_US |
| dc.language.iso | English | en_US |
| dc.pages | 142 | en_US |
| dc.publisher | SRI VENKATESWARA VETERINARY UNIVERSITY TIRUPATI - 517 502. (A.P.) INDIA | en_US |
| dc.sub | Veterinary Pharmacology and Toxicology | en_US |
| dc.theme | STUDIES ON THE HEPATOPROTECTIVE EFFECT OF SARDINELLA LONGICEPS FISH EXTRACT AND FLAVONOID QUERCETIN IN RATS | en_US |
| dc.these.type | M.V.Sc. | en_US |
| dc.title | STUDIES ON THE HEPATOPROTECTIVE EFFECT OF SARDINELLA LONGICEPS FISH EXTRACT AND FLAVONOID QUERCETIN IN RATS | en_US |
| dc.type | Thesis | en_US |