ISOLATION AND CHARACTERIZATION OF ENTOMOPATHOGENIC FUNGI AND THEIR EFFECTIVENESS
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Date
2007-08-30
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University of Agricultural Sciences GKVK, Banglore
Abstract
An attempt was made to isolate and characterize native
entomopathogenic fungi. Nine insect mycopathogens, belonging to
seven genera were found naturally occurring in the nine districts of
Northern Karnataka. Morphological variation among the field collected
isolates of M. anisopliae (Ma2) and V. lecanii (VI1) for various
parameters like, hyphal and conidial characters, sporulation and
spore yield were recorded. Pathogenicity of V. lecanii to aphids,
whitefly and mites and that of M. anisopliae to Americain bollworms
£ind rhinocerous beetle was proved in laboratory. Among different
carbon sources tested, starch proved superior for both the
mycopathogens NH4 (SO4) and KNO3 were superior as nitrogen source.
Bajra and rice grains served as most productive media for conidial
growth of Ma2 and Vll with an yield of 22.77 x 10^ and 24.59 x 10®
conidia per g of media, respectively. Among pesticides tested,
fungicides showed maximum inhibition followed by insecticides and
weedicides. The persistence of mycopathogens (Ma2 and Vll) was
higher in soil (upto 16 months) than in phylloplane (upto 3 months).
In general, all the carrier material had more conidial viability when
stored in refrigerated and deep freezer condition and a reduction of
more than 10 per cent and 8 per cent in refrigerated and deep fereezer
was observed after 150 DAS respectively. Oil formulations proved
better than wettable powder.
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